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Role of microtubules and related proteins on cellular function

Research Project

Project/Area Number 60300007
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionUniversity of Tokyo

Principal Investigator

MABUCHI Issei (1986)  University of Tokyo/Associate Professor, 教養部, 助教授 (40012520)

酒井 彦一 (1985)  東京大学, 理学部, 教授

Co-Investigator(Kenkyū-buntansha) SOBUE Kenji  Oosaka University/Associate Professor, 医学部, 助教授 (20112047)
WATANABE Yoshio  Tsukuba University/Professor, 生物科学系, 教授 (00015918)
KUROKAWA Masanori  University of Tokyo/Professor, 医学部, 教授 (90009902)
SATO Hidemi  Nagoya University/Professor, 理学部, 教授 (40109260)
HIRAMOTO Yukio  Tokyo Institute of Technology/Professor, 理学部, 教授 (50011440)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥16,000,000 (Direct Cost: ¥16,000,000)
Fiscal Year 1986: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1985: ¥10,000,000 (Direct Cost: ¥10,000,000)
KeywordsMicrotubule / Cytoskeleton / Actin / Chromosome Movement / Axonal Transport / Nerve Excitation / 中間径繊維
Research Abstract

The purpose of this co-operative research project has been to clarify the mechanism of regulation of various cellular functions by cytoskeletons. There were six subprojects. 1. Function of microtubule-dynein system in mitosis The motive force for the chromosome movements was localized in the half spindle region near chromosomes. The formation of barrel-shaped spindle in the presence of T-1 was thought to be a result of scattering of the microtubule-organizing granules by T-1. MAP1 was localized on the cytoskeletal microtubules at <G_0> stage of cultured cells while it became concentrated in the nucleus at <G_1> stage. 2. Cytoskeletal proteins and cell motility Microtubules were found to undergo spontaneous assembly-disassembly cycle by dark field microscopy. Intermediate filaments were found to be relevant to mitosis and exchange of nuclei after conjugation in Tetrahymena. Complete amino acid sequences of Tetrahymena actin and of -tubulin from lymphoma cells were determined. 3. Dynamic state of microtubules in axons It was found that the 200K subunit of neurofilament is phosphorylated and transported more faster than the bulk neurofilament proteins. Axolinin was found to co-exist with microtubules in squid giant axon. 4. Function of cytoskeleton-modulating proteins A 100K-molecular weight Casensitive actin filament-severing protein was found in sea urchin eggs. Alphaactinin was found to be concentrated in the cortex of the fertilized sea urchin egg together with actin filaments. 5. Nerve excitability and microtubules Calmodulin antagonists were found to interfere with Na-current in squid nerve. 6. Cytoskeleton and cellular function Ultrastructure of the plasma membrane undercoat in skeletal muscle and astrocytes where myofibrils and intermediate filaments, respectively, attach was visualized.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (64 results)

All Other

All Publications (64 results)

  • [Publications] 高橋大輔: Cell Structure and Function. 10. 327-337 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 室伏拡: Cell Motility and the cytoskeleton. 6. 83-88 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 月田承一郎: Journal of Cell Biology. 102. 1710-1725 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 細谷浩史: European Journal of Biochemistry. 154. 233-239 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 倉持健太郎: Biomedical Research. 7. 65-68 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 大沼雅明: Journal of Biochemistry. 100. 817-820 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 須藤和夫: Biochemistry. 25. 6186-6192 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 細谷浩史: FEBS Letters. 205. 121-126 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 浜口幸久: Cell Motility and the Cytoskeleton. 6. 549-559 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 浜口みやこ: Development, Growth and Differentiation. 28. 143-156 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 浜口みやこ: Development,Growth and Differentiation. 28. 461-470 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 五十嵐道弘: FEBS Letters. 192. 239-242 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 五十嵐道弘: Journal of Neurochemistry. 47. 1720-1727 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 小宮義璋: Biomedical Research. 7. 345-348 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 小宮義璋: Biomedical Research. 7. 359-363 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 伊藤知彦: Zoological Science. 3. 255-264 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 大原たかね: Development,Growth and Differentiation. 28. 369-373 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 大場弘義: Journal of Biochemistry. 100. 797-808 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 広野雅文: Journal of Molecular Biology. 193. I . (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 松岡達巨: Zoological Science. 3. 437-443 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 西孝子: Journal of Experimental Zoology. 239. 175-182 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 松岡達臣: Archives Protistenkd.131. 85-94 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 柿本辰男: Plant and Cell Physiology. 27. 91-101 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 原田文子: Experimental Cell Research. 166. 265-278 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 佐藤周子: Experimental Cell Research. 167. 281-286 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 祖父江憲治: Proceedings of the National Academy of Science U.S.A.84. I P . (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 小林孝彰: Journal of Cell Biology. 102. 1699-1709 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 馬渕一誠,酒井彦一,毛利秀雄,佐藤英美,秦野節司,渡辺良雄,矢原一郎,佐藤周子,祖父江憲治,新井孝夫: "続・生化学実験講座(細胞骨格の構造と機能)" 東京化学同人, 636 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 平本幸男: "実験生物学構座11<発生物物学>" 丸善, (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 浜口幸久,平本幸男,秦野節司,黒川正則,重中義信,柴岡弘郎,矢原一郎: "図説・細胞骨格" 講談社サイエンティフィク, 213 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 平本幸男: "Methods in Cell Biology" Academic Press, (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 祖父江憲治: "現代皮膚学体系" 中山書店, (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroshi Hosoya, Issei Mabuchi and Hikoichi Sakai: "An 100-kDa <Ca^(2+)> -sensitive actin-fragmenting protein from unfertilized sea urchin egg." European Journal of Biochemistry. 154. 233-239 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kentaro Kuramochi, Issei Mabuchi and Kastushi Owaribe: "Spectrin from sea urchin eggs" Biomedical Research. 2. 65-68 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohnuma and Issei Mabuchi: "The 45K Molecular Weight Actin-Modulating Protein from Sea Urchin Eggs Forms a Complex with Actin in the Presence of Calcium Ions." Journal of Biochem. 100. 817-820 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kazuo Sutoh and Issei Mabuchi: "Improved method for mapping the binding site of an actin-binding protein in the actin sequence. Use of a site-directed antibody against the N-terminal region of actin as a probe of its N-terminus." Biochmistry. 25. 6186-6192 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroshi Hosoya, Fuyuki Iwasa, Masaaki Ohnuma, Issei Mabuchi, Hideo Mohri, Hikoichi Sakai and Yukio Hiramoto: "A novel 15kDa <Ca^(2+)> -binding protein present in the eggs of the sea urchin Hemicentrotus pulcherrimus." FEBS Letters. 205. 121-126 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yukihisa Hamaguchi and Issei Mabuchi: "Alpha-actinin accumulation in the cortex of echinoderm eggs during fertilization." Cell Motility and the Cytoskeleton. 6. 549-559 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Miyako S. Hamaguchi and Yukio Hiramoto: "Analysis of the role of astral rays in pronuclear migration in sand dollar eggs by the colcemid-UV method." Develop, Growth and Differentiation. 28. 143-156 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Miyako, S. Hamaguchi, Yukihisa Hamaguchi and Yukio Hiramoto: "Microinjected polystylene beads move along astral rays in sand dollar eggs." Development, Growth and Differentiation. 28. 461-470 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Michihiro Igarashi, Yoshiaki Komiya and Masanori Kurokawa: "CMP-sialic acid, the sole sialosyl donor, is intra-axonally transported." FEBS Letters. 192. 239-242 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Michihiro Igarashi, Yoshiaki Komiya and Masanori Kurokawa: "A ganglioside species ( <G_(DIalpha)> migrates at a slow rate and CMP-sialic acid severalfold faster in Xenopus sciatic. nerve: Fluorographic demonstration." Journal of Neurochemistry. 47. 1720-1727 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yoshiaki Komiya, Tomoko Tashiro and Masanori Kurokawa: "Biomedical Research" Phosphorylation of neurofilament proteins during their axonal transport.7. 345-348 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yoshiaki Komiya, Tomoko Tashiro and Masanori Kurokawa: "Occurrence of faster migrating neurofilament proteins as revealed by their phosphorylation." Biomedical Research. 7. 359-363 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Tomohiko J. Itoh, Hidemi Sato and Akio Kobayashi: "T-1 dependent barrel-shaped induction in the sea urchin eggs and the role of T-1 in microtubule assembly." Zoological Science. 3. 255-264 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Takane Ohara and Hidemi Sato: "Distributional change of membrane-associated calcium in sea urchin eggs during fertilization." Development , Growth and Differentiation. 28. 369-373 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroyoshi Ohba, Ikuyo Ohmori, Osamu Numata and Yoshio Watanabe: "Purification and immunofluorescent localization of the mutant gene product of a Tetrahymena cdaAl mutant affecting cell division." Journal of Biochemistry. 100. 797-808 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masafumi Hirono, Hiroshi Endoh, Norihiro Okada, Osamu Numata and Yoshio Watanabe: "Tetrahymena actin. Cloning and sequencing of the Tetrahymena actin gene and identification of its gene product." Journal of Molecular Biology. 193. In press. (1987)

    • Description
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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Tatsuomi Matsuoka, Katsuyoshi Ishigame, Yojiro Meneoka and Yoshinobu Shigenaka: "External factors which induce the rapid contraction of Heliozoan axopodia." Zoological Science. 3. 437-443 (1986)

    • Description
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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Takako Nishi, Makoto Kobayashi and Yoshinobu Shigenaka: "Membrane activity and its correlation with vacuolar contraction in the Heliozoan Echinosphaerium." Journal of Experimental Zoology. 239. 175-182 (1986)

    • Description
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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Tatsuomi Matsuoka and Yoshinobu Shigenaka: "Elongation and contraction of Blepharisma, evoked by mechanical or light stimulation." Archives Protistenkd.131. 85-94 (1986)

    • Description
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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Tatsuo Kakimoto and Hiroh Shibaoka: "Calcium-sensitivity of cortical microtubules in the green alga Mougeotia" Plant and Cell Physiology. 27. 91-101 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Fumiko Harada, Fumio Matsuzaki and Ichiro Yahara: "Occurrence of two -tubulin isoforms with different polymerizing abilities in L5178Y cells." Experimental Cell Research. 166. 265-278 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Chikako Sato, Kimiko Nishizawa, Tokiko Nakayama, Reiko Hirai and Hiromu Nakamura: "Phosphorylated 350kD protein in the nucleus as it is associated with cell transformation." Experimental Cell Research. 167. 281-286 (1986)

    • Description
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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kenji Sobue, Toshio Okabe, Kozo Kadowaki, Kazuyuki Itoh, Toshihiro Tanaka and Yasushi Fujio: "Cytosynalin: A Mr 35,000 cytoskeleton-interacting and calmodulin-binding protein." Proceedings of the National Academy of Science, U. S. A.84. In press. (1987)

    • Description
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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Takaaki Kobayashi, Shoichiro Tsukita, Sachiko Tsukita, Yoshio Yamamoto and Gen Matsumoto: "Subaxolemmal cytoskeleton in squid giant axon. <I> Biochemical analysis of microtubules, microfilaments, and their associated high-molecular weight proteins." Journal of Cell Biology. 102. 1699-1709 (1986)

    • Description
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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Daisuke Takahashi, Hiromu Murofushi, Koichi Ishiguro, Jun Ikeda and Hikoichi Sakai: "Phosphoprotein phosphatase inhibits flagellar movement of Triton models of sea urchin spermatozoa." Cell Structure and Function. 10. 327-337 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiromu Murofushi, Koichi Ishiguro, Daisuke Takahashi, Jun Ikeda and Hikoichi Sakai: "Regulation of sperm flagellar movement by protein phosphorylation and dephosphorylation." Cell Motility and the Cytoskeleton. 6. 83-88 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Shoichiro Tsukita, Sachiko Tsukita, Takaaki Kobayashi and Gen Matsumoto: "Subaxolemmal cytoskeleton in squid giant axon. <II> . Morphological identification of microtubule- and microfilament-associated domains of axolemma." Journal of Cell Biology. 102. 1710-1725 (1986)

    • Description
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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Issei Mabuchi, Hikoichi Sakai, Hideo Mohri, Hidemi Sato, Sadashi Hatano, Yoshio Watanabe, Ichiro Yahara, Chikako, Sato, Kenji Sobue, Takao Arai: Tokyo Kagaku Dojin. Zoku Seikagakujikken Kouza, 636 (1985)

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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yukihisa, Hamaguchi, Yukio Hiramoto,-Sadashi Hatano, Masanori Kurokawa, Yoshinobu Shigenaka, Hiroh Shibaoka, Ichiro Yahara: Koudansha Scientific. Zusetsu Saiboukokkaku, 213 (1985)

    • Description
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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yukio Hiramoto: Academic Press, Inc.Methods in Cell Biology, (1986)

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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yukio Hiramoto: Maruzen. Jikken Seibutsugakukoza 11 [Hassei Seibutsugaku], (1985)

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    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kenji Sobue: Nakayamashoten. Gendai Hifugakutaikei,

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    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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