| Project/Area Number |
60300011
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
広領域
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| Research Institution | Niigata University |
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Principal Investigator |
TAKAHASHI Yasuo Niigata University, Brain Research Institute, Professor, 脳研究所, 教授 (00018590)
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| Co-Investigator(Kenkyū-buntansha) |
MITSUI Hiromi Niigata University, School of Science, Professor, 理学部, 教授 (90109942)
FUJITA Tsuneo Niigata University, School of Medicine, Professor, 医学部, 教授 (00032863)
ISOBE Toshiaki Tokyo Metropolitan University, Faculty of Science, Research Associate, 理学部, 助手 (70106607)
UYEMURA Keiichi Saitama Medical School, Professor, 医学部, 教授 (90049792)
TSUKADA Yasuzo Keio University, School of Medicine, Professor, 医学部, 教授 (00050956)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1985: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | Brain / Protein / Gene / DNA / ONA / RNA / DNA |
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| Research Abstract |
Our co-operative research group attempted to characterize several brain-specific proteins using recombinant DNA technique and to apply this technique for neurohistological study. We succeeded in cloning of genomic DNA for neuron-specific enolase, S-100 , cholecystokinin and carried out their gene expression in vitro and in vivo. In addition, we succeeded in cloning of cDNA for bovine and human 2',3'-cyclic nucleotide 3'-phosphodiesterase, 14-3-3 protein (tyrosine gydroxylase activator) and enolase and determined their nucleotide sequences. Furthermore, we determined the complete amino acid sequence fo bovine peripheral myelin PO protein. Fujita, one of our group members, applied cDNA of several proteins for examining intra-cellular localization of their mRNA using in situ gybridization.
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