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Bacterial differentiation: Its application on production of useful substances.

Research Project

Project/Area Number 60303024
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 発酵・醸造
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

KOBAYASHI Yasuo  Hiroshima Univ., Fac. Appl. Biol. Sci., 生物生産学部, 教授 (10013319)

Co-Investigator(Kenkyū-buntansha) SADAIE Yoshito  Natl. Inst. Genet., 助教授 (40000252)
YAMANE Kunio  Univ. of Tsukuba, Inst. Biol. Sci., 生物科学系, 助教授 (20013336)
YAMASAKI Makari  Univ. of Tokyo, Fac. Agr., 農学部, 教授 (60011889)
BEPPU Teruhiko  Univ. of Tokyo, Fac. Agr., 農学部, 教授 (80011873)
SAITO Hiuga  Univ. of Tokyo, Inst. Appl. Microiol., 応用微生物研究所, 教授 (10013301)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1985: ¥4,300,000 (Direct Cost: ¥4,300,000)
KeywordsBacillus subtilis / sporulation / catabolite repression / A-factor / promoter-probe vector / secretion vector / gene amplification / プテアーゼ産生 / 枯草菌胞子形成初期反応 / 胞子形成初期遺伝子【_(spo)OA】 / 好アルカリ性バチルス / 翻訳共役 / 放線菌化学調節物質
Research Abstract

1. Control mechanism of bacterial differentiation (Saito, Sadafe, Fujita, Ikeuchi, Kawamura) (1) Regulatory mechanism of sporulation initiation gene spoOA expression in Bacillus subtilis was studied by the use of lacZ-fusion gene and temperature-sensitive spoOA mutant. It was shown that expression of spoOA gene depends on spoOA, OB, OE, OF, and OH genes and spprulation was not initated unless the function of SpoOA protein is derepressed. (2) spoOA gene was cloned and its gene product was overproduced in Escherichia coli and purified. Purified SpoOA protein has DNA-binding activity. (3)div gene which regulates cell division, sporulation, and protein secretion was cloned. (4) Catabolite-repressible gluconate operon was cloned. The whole nucleotide sequence was determined and the mechanism of catabolite repression of B. subtilis was studied.
2. Isolation of regulatory factor of gene expression (Beppu, Nakayama) (1) Streptomycin production and sporulation in Streptomyces griseus is positively controlled by A-factor. A-factor was purified and regulatory network surrounding A-factor was studied. (2) Chromosomal proteins of B. subtilis were isolated and purified and sporulation-specific chromosomal proteins were identified.
3. Development of usefulvector (Kobayashi, Yamasaki, Yamane, Kudoh, Tanaka) (1) Isolation and analysis of a temperature-dependent promoter revealed that its expressio is controlled by translational-coupling. Stable promoter-probe vector for B. subtilis was constructed. (2) A commom method to amplify a useful gene in B. Subtilis chromosome was developed. (3) Secretion vector carrying -amylase signal peptide was constructed and improvement of its secretion ability was intended. (4) prtR gene which increases protease production in B. natto was identified and cloned. It was shown that PrtR protein is a positive factor which stimulates the transcription of protease gene.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Yamashita,S.: Mol. Gen. Genet.205. 28-33 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mori, M.: E-tmrB region. J. Bacteriol.166. 787-794 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nakazawa, K.: Biochem. Biophys. Res. Commun.134. 624-631 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kudoh, J.: Proc. Natl. Acad. Sci. U. S. A.82. 2665-2668 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Fukumori, F.: FEMS Microbiol. Lett.48. 65-68 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nagami, Y.: J. Bacteriol.166. 20-28 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tanaka, T. ed.by Ganesa, A. T., and Hoch, J. A.: "Bacillus Molecular Genetics and Biotechnology Applications" Academic Press, New York, 496 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Horinouchi, S. ed. by Holt, G., and Saunders, G.: "Genetic Modification of Industrial Microorganisms" Plenum Press, London, (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Horinouchi, S.: "Nucleotide sequence of the streptothricin acetyltransferase gene from Streptomyces lavendulae and its expression in heterologous hosts." J. Bacteriol.169. 1929-1937 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mori, M.: "Designed gene amplification on the Bacillus subtilis chromosome." J. Gen. Microbiol.(1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Fujita, Y.: "The gluconate operon gnt of Bacillus subtilis encodes its own transcriptional negative regulator." Proc. Natl. Acad. Sci. U. S. A.84. 4524-4528 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Miwa, Y.: "Efficient utilization and operation of the gluconate-inducible system of the promoter of the Bacillus subtilis gnt operon in Escherichia coli." J. Bacteriol.169. 5333-5335 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ikeuchi, T.: "Purification and characterization of the SpoOA protein of Bacillus subtilis from and overproducing strain of Escherichia coli." Eur. J. Biochem.167. 233-238 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kobayashi, Y. (ed by B. Maruo & H. Yoshikawa): Bacillus subtilis - Molecular Biology and Its Industrial Application. Kohdansha Scientific, (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirofumi Yoshikawa: Nucleic Acids Res.14. 1063-1072 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Masanao Oda: Agric.Biol.Chem.50. 2845-2852 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Shinya Yamashita: Mol.Gen.Genet.205. 28-33 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yoshikatsu Murooka: 24. 499-503 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yoshikatsu Murooka: Appl.Microbiol.Biotechnol.24. 504-508 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yasutaro Fujita: J.Gen.Microbiol.132. 161-169 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yasutaro Fujita: Nucleic Acids Res.14. 1237-1252 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yasutaro Fujita: J.Biol.Chem.261. 13744-13753 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yasutaro Fujita: Proc.Natl.Acad.Sci.U.S.A.

    • Related Report
      1986 Annual Research Report
  • [Publications] Toshihiko Ikeuchi: Mol.Gen.Genet.203. 371-376 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] S.Horinouchi: J.Bacteriol.168. 257-269 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Nakazawa: Biochem Biophys.Res.Commun.134. 624-631 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Furusato: J.Bacteriol.165. 549-556 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Furusato: J.Biochem.99. 1181-1190 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Furusato: Agric.Biol.Chem.50. 1771-1775 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Takano: J.Bacteriol.166. 1118-1122 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Higashiguchi: Mol.Gen.Genet.204. 36-43 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Kimura: Appl.Microbiol.Biotechnol.

    • Related Report
      1986 Annual Research Report
  • [Publications] O.Kurahashi: Agric.Biol.Chem.

    • Related Report
      1986 Annual Research Report
  • [Publications] F.Fukumori: J.Gen.Microbiol.132. 2329-2335 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] F.Fukumori: J.Bacteriol.168. 479-485 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] F.Fukumori: FEMS Microbiol.Lett.40. 311-314 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 堀之内未治: "抗生物質研究の最先端" 東京化学同人, (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 中山建男: "生物化学実験法「クロマチン実験法」" 学会出版センター,

    • Related Report
      1986 Annual Research Report
  • [Publications] 山根國男: "「アミラーゼ」-生物工学へのアプローチ" 学会出版センター, (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 山根國男: "「細胞表層」構造と機能を探る" 学会出版センター, (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Yamane: "Methods in Enzymology" Academic Press,Inc.,New York,

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Yamane: "Bacillus Molecular Genetics and Biotechnolgy Application" Academic Press,Inc.,New York, (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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