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Application of new technologies to rice breeding

Research Project

Project/Area Number 60304016
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionTamagawa University (1986)
The University of Tokyo (1985)

Principal Investigator

NAKAJIMA Tetsuo  Faculty of Agriculture, Tamagawa University, Professor, 農学部, 教授 (50012026)

Co-Investigator(Kenkyū-buntansha) HINATA Kokichi  Faculty of Agriculture, Tohoku University, Professor, 農学部, 教授 (00005589)
OMURA Takeshi  Faculty of Agriculture, Kyushu University, Professor, 農学部, 教授 (40038170)
FUTSUHARA Uzo  Faculty of Agriculture, Nagoya University, Professor, 農学部, 教授 (70023405)
HIRAI Atsushi  Faculty of Agriculture, Nagoya University, Associate Professor, 農学部, 助教授 (60023470)
TANAKA Kunisuke  Faculty of Agriculture, University of Kyoto Prefecture, Associate Professor, 農学部, 助教授 (90027194)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥8,800,000 (Direct Cost: ¥8,800,000)
Fiscal Year 1986: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1985: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordsrice breeding / storage protein / chloroplast DNA / mitocondorial DNA / plant regeneration / anther culture; protoplast / プロトプラスト / 半矮性
Research Abstract

For the application of new technologies such as cell fusion and gene manipulation to rice breeding, it is necessary to accumlate the knowledges of molecular level in rice plants and also to improve the technologies in tissue culture. After getting gene-manipulated plants, an efficient selection system for improving to a new variety should be required.
1. The cDNA library on poly <(A)^+> RNA which related to the synthesis of rice storage protein, glutelin and prolamin, was established. It was clearified that the storage protein in endosperm formed two kinds of granules, protein body <I> and <II> , and nondigestable protein body <I> mainly consisted prolamin.
2. A physical map of rice chloroplast DNA was established and the location of the genes for large subunit of Rubisco and the subunits of ATPase was proved. The base sequences of the genes were also determined. On the mitocondorial DNA, cloning of all DNA fragments was succeeded and a fragment having activity of autonomously replicating sequence was found out.
3. Adventive shoot formation and somatic embryogenesis from callus induced from seeds and pollen were investigated using many varieties, and genotypic variability in plant regeneration was clearified. On the basis of the results, plant regeneration was succesively induced from protoplasts originated from seed callus.
4. Characteristics of mutator gene obtained from progenies of slender grain mutant was analyzed and strong effect of the gene as amutagen was clearified. New sugary and shrunken mutants were obtained by treating fertilized egg cells with nitroso compounds.
5. Gene expression of semi-dwarfness of Dee-geo-woo-gen type was investigated in internode elongation, and also phenotypic expression of drought resistance and cooking quality were analyzed in their related characters.

Report

(1 results)
  • 1986 Final Research Report Summary

Research Products

(20 results)

All Other

All Publications

  • [Publications] Furuta,Masaichi: Plant Cell Physiol.27. 1201-1204 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Sugimoto,T.: Agric.Biol.Chem.50. 3031-3035 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hirai,A.: Theor.Appl.Genet.72. 117-122 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 平井篤志: 育種学雑誌. 37(別1). (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Abe,T.: Theor.Appl.Genet.72. 3-10 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Abe,T.: Japan.J.Breed.36. 1-6 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 朱政治: 育種学雑誌. 36(別1). 66-67 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 石井千津: 育種学雑誌. 36(別2). 220-221 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 山縣弘忠: 育種学雑誌. 37(別1). (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 山縣弘忠: 育種学雑誌. 37(別1). (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Omura,T.: "Rice genetics"Proceeding of the international rice genetics symposium.IRRI.707-717 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Fukoshima,M.T.: Japan.J.Breed.35. 109-117 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Fukoshima,M.T.: Japan.J.Breed.35. 292-300 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 大場伸哉: 育種学雑誌. 36(別2). 312-313 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Furuta,Masaichi: "Cell-free synthesis of the rice glutelin precurser." Plant Cell Physiol.27. 1201-1204 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Sugimoto, T.: "Molecular species in the protein body <II> (PB- <II> ) of developing rice endosperm." Agri. Biol. Chem.50. 3031-3035 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hirai,Atsushi: "Rice chloroplast DNA; a physical map and the location of the genes for the large subunit of ribulose 1,5- biphosphate carboxylase and the 32KD photosystem <II> reaction center protein." Theor. Appl. Genet.72. 117-122 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Abe, t.: "Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.)" Theor. Appl. Genet.72. 3-6 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Omura,T.: "Mutagenesis in rice by treating fertilized egg cells with nitroso compound." "Rice Genetics" Proceeding of the internationasl rice genetics symposium. IRRI.707-717 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Fukoshima,M.T.: "Varietal comparison on the responses of photosynthetic rates and leaf water balance at different soil moisture tensions in rice." Japan.J.Breed.109-117 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-30   Modified: 2016-04-21  

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