| Project/Area Number |
60304019
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
作物
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| Research Institution | Faculty of Agriculture, Nagoya University. |
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Principal Investigator |
MAEDA Eizo Faculty of Agriculture, Nagoya University., Prof., 農学部, 教授 (60023404)
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| Co-Investigator(Kenkyū-buntansha) |
FURUHASHI KATSUHISA Faculty of Science, Niigata University., 理学部, 助教授 (90022714)
MIYAKE Hiroshi Faculty of Agriculture, Tokyo Agr. Technol. University., 農学部, 助手 (60134798)
ISHIHARA Aiya Faculty of Agriculture, Iwate University., 農学部, 教授 (20011827)
YAMAGUCHI Toshihiko Faculty of Agriculture, Osaka Pref. University., 農学部, 講師 (30081558)
TANIGUCHI Takeshi Facultu of Agriculture, Nagoya University., 農学部, 助教授 (10023409)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1985: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | activity of ADH / aged seed / centripetal disposition of chloro-plast / cytochalasin B / embryoid / nucellus culture / rice scutellum / 胚盤 / 組織培養 / 不定胚形成 / 老化種子 / 葉緑体の移動 / 亜鉛濃度 / アミノ酸 / 胚盤の微細構造 |
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| Research Abstract |
Induction and the development of shoots, roots and somatic embryos was studied with morphology, ultrastructure and biochemistry. (1) Successuful germination of aged seeds was made by ABA and brassinolode. Embryoids were developed from millet seeds and subcultured resulting in plantlet formation. (2) Somatic embryos arose throught nucellus culture, anther culture and leaf segment culture of Malus. (3) Centripetal disposition of chloroplasts, mitochondria and microbodies was occurred in the bundle sheath cells during the development of Eleusine coracana leaves and its movement was inhibited by auxin, cycloheximide and cytochalasin B. (4) The activity of ADH in rice callus increased in the process of dedifferentiation. (5) When supplied from rice scutellum, IAA, ABA and brassinolide accelerated the seminal root growth but not from root medium.
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