| Project/Area Number |
60304021
|
|---|
| Research Category |
Grant-in-Aid for Co-operative Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
植物保護
|
|---|
| Research Institution | Faculty of Agriculture, Tokyo University of Agriculture and Technology |
|---|
Principal Investigator |
AOKI Joji Faculty of Agriculture, Tokyo University of Agric & Technol. (Prof.), 農学部, 教授 (30014905)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHOBA Michio Faculty of Agriculture, Kyushu University (Assis. Prof.), 農学部, 助手 (80038281)
KAWARABATA Isamu Faculty of Agriculture, Kyushu University (Assoc. Prof.), 農学部, 助教授 (60038221)
AIZAWA Keio Faculty of Agriculture, Kyushu University (Prof.), 農学部, 教授 (00038142)
HUKUHARA Toshihiko Faculty of Agric. Tokyo University of Agr. & Tech. (Assoc. Prof.), 農学部, 助教授 (70011880)
IWAHANA Hidenori Faculty of Agric. Tokyo University of Agr. & Tech. (Assoc. Prof.), 農学部, 助教授 (90038240)
|
|---|
| Project Period (FY) |
1985 – 1986
|
| Project Status |
Completed (Fiscal Year 1986)
|
| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | Serious insect pest / Microbial control / Insect pathogen / Selection of high pathogenic strain / Detection of insect virus / Strain improvement / Molecular genetics of insect pathogen |
|---|
| Research Abstract |
Highly insect-pathogenic microorganisms were selected by bioassay and field investigations as follows : Erynia radicans and Entomophthora planchoniana to several species of the aphid, Metarhizium anisopliae and Beauveria bassiana to ricewater weevil, Serratia marcescens to peach fruit moth, 3 strains of nuclear polyhedrosis viruses to common cutworm, B. bassiana, B. brongniartii, B. amorpha, and M. anisopliae to several species of the beetle. Some of these microorganisms were investigated on their physiological and biochemical properties. All strains of Bacillus thuringiensis which showed high toxicity to the house fly produced heat-stable toxin. Polyhedral inclusion bodies of a nuclear polyhedrosis virus of the yellow-legged tussock moth were detected in field soil by immunofluorescence microscopy. Cloning and transformation of a plasmid including toxin-gene were conducted in a strain of Bacillus thuringiensis. Strain improvement of insect-pathogenic microorganisms was also examined by shotgun sequence of DNA.
|
