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Studies on synthesis of ribosomes and its regulation of higher animals

Research Project

Project/Area Number 60304047
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionNiigata University

Principal Investigator

OGATA Kikuo  Niigata university Aonorary, Profeesor, 名誉教授 (00018285)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Tatsuo  Miyazaki Medical School associate professor, 医学部, 助教授 (60031712)
TSURUGI Ikunio  Yamanashi Medical School, Professor, 医学部, 教授 (10018690)
TANAKA Tatsuo  Yamagata University, associate professor, 医学部, 助教授 (70018688)
MITSUI Hiromi  Niigata University, Professor, 理学部, 教授 (90109942)
MURAMATSU Masami  The University of Tokyo, Professor, 医学部, 教授 (10035454)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 1987: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1986: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1985: ¥4,000,000 (Direct Cost: ¥4,000,000)
Keywordsribosome / rRNA / ribosomal protein / rDNA promoter / point mutation / リボゾーム蛋白 / rDNA / 転写開始因子 / T細胞増殖制御因子 / セレクター
Research Abstract

To investigate the mechanisms of synthesis of ribosomes in higher animals, following projects were performed. (1)cDNA clones specific for rat ribosomal proteins were isolated and analyzed to determine the primary amino acids sequences.Genomic clones hybridized with these cDNA clones were preparing.(Ogata and Tanaka) (2)By using point mutations introduced into mouse rDNA promoter region and chimeric rDNA between mouse and human,the abotrscriptniton and speciesdepndc TRANSCRIPTION WERE INVESTIGATED.(MURAMATSU) (3)A NEW PROLIDERATING FACTOR(TGF-BF) WAS PURIFIED FROM MOUSE BONE MARROW CELL TRANSFECTED WITH ADENOVIRUS E. THE EFFECT OF THIS FACTOR ON TRANSCRIPTION WAS EXAMINED. (MITSUI) (4)AN ANTIBODY AGAINST ACIDIC PROTEIN (A1/A2) OF YEAST RIBOSOMES WERE PREPARED AND EXAMINED THE POOL SIZE AND LOCALIZATION OF A1/A2.TRANSPORTATION OF RIBOSOMAL PROTEINS FROM CYTOPLASM TO NUCLEUS WAS INVESTIGATED BY USING THE METHOD OF MICROINJECTION OF XENOPUS LAEVIS. (TSURUGI) (5)THE METHOD OF SEPARATING THE CHROMATIN PROTEIN OF RAT LIVER WAS ESTABLISHED BY USING REVERSE PHASE HIGH PERFORMNCE LIQUID CHROMATOGRAPHY(HPLC), which was used to purify "selector" of ribosomal protemn genes. (Nakayama) (6)An in vitro processing system was developed by using RNA polymerase I-specific or bacteriophage SP6 transcription system to investigate the molecular mechanism of mammalian rRNA precursor. By using this system, protein factor and nucleotide sequence specified for the processing were identified. (Mishima)

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] T.Tanaka: J.Biol.Chem.260. 6329-6333 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Kuwano: J.Biochem.97. 983-992 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.Tanaka: Eur.J.Biochem.154. 523-527 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Kato,: Mol. Cell. Biol.6. 3418-3427 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.Nagamine: Mol.Cell.Biol.7. 1486-1495 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Tsurugi: J.Biochen.98. 1427-1431 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Tsurugi: J.Biochem.99. 237-241 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Tsurugi: Exp.Cell Res.174. 177-187 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.Nakayama: Mol.Gen.Genet.201. 252-257 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Tamura,: Eur.J.Biochem.168. 83-87 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Mishima: The EMBO Journal. 4. 3879-3889 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Mishima: J.Biochem.98. 279-287 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 村松正実: "新基礎学生化学実験法7 遺伝子工学" 中嶋暉躬.野本明男.松橋通生.三浦謹一郎.村松正実 編(丸善株式会社), 297 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 村松正実: "新生理科学大系 第6巻 細胞内小器官の生理学" 田代裕.水上茂樹(医学書院),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Tamura: "Molecular cloning and nucleotide sequence of cDNA specific for rat ribosomal protein L5" Eur.J.Biochem.168. 83-87 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.nagamine: "Sequestration analysis for RNA polymerase Itranscriptio factors with various deletion and point mutations reveals different functional regions of the mouse rRNA gene promoter" Mol.Cell.Biol.7. 1486-1495 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.Kuzumaki: "Rat ribosomal protein L3ka multigene family:molecular structure and characterization of three L3ka-related pseudogenes" Biochim.Biophys.Acta. 909. 99-106 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Mitsui: "Chracterization of a novel acidic protein of 38 KDa,AO, in yeast ribosomes which immunologically cross-reacts with the 13 KDa acidic ribosomal proteins,A1/A2" J.Biochem.102. 1565-1570 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Tsurugi: "The metabolism of ribosomal proteins microinjected into the oocytes of Xenopus laevismmunologically cross-reacts with the 13 KDa acidic ribosomal proteins,@" Exp.Cell Res.174. 177-187 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 緒方規矩雄: 新潟医学会雑誌. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Kato;M.Nagamine;R.Kominami;M.Muramatsu: Mol.Cell.Biol.6. 3418-3427 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Tanaka;K.Wakasugi;Y.Kuwano;K.Ishikawa;K.Ogata: Eur.J.Biochem.154. 523-527 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Tanaka;Y.Kuwano;T.Kuzumaki;K.Ishikawa;K.Ogata: Eur.J.Biochem.in press. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Tsurugi;K.Ogata: J.Biochem.99. 237-241 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Nakayama;M.Irikura;Y.Setoguchi;M.Nakayama;M.Mochizuki;K.Ogata: Mol.Gen.Genet.201. 252-257 (1985)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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