| Project/Area Number |
60304052
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | Kyushu University |
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Principal Investigator |
AMAKO Kazunobu Kyushu University ,Faculty of Medicine,Professor, 医学部, 教授 (20078752)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAE Taiji Tokai University, Faculty of Medicine , Professor, 医学部, 教授 (50102851)
TAKADA Haruhiko Ookasa University, Faculty of Dentistry, Lecture, 歯学部, 講師 (30135743)
KAWATA Tomio Tokushima University, Faculty of Medicine , Professor, 医学部, 教授 (60035368)
KANEMASA Yasuhiro Okayama University, Faculty of Medicine, Professor, 医学部, 教授 (80330059)
KATO Keijiro Okayam University, Faculty of Dentistry, Professor, 歯学部, 教授 (50028718)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1987: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1986: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1985: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | Cell surface / Virulence / fimbriae / colonization / invasion / muramyl dipeptide / 侵入性 / 付着因子 / 表層構造 / 侵入因子 / 線毛 / α毒素 / リポタイコ酸 / ミコール酸 / 生理活性物質 |
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| Research Abstract |
The works of the member in this project can be categorized into three different experimental groups, the group on the colonization factor, on the biological activity of cell surface materiales and on the invasing mechanisms. The experimental results of these groups in these three years are summerized as follow.
The group of the colonization factors: From several species of bacteria fimbrial colonization factors were isolated, purified and the chemical compositions were analysed. In E. coli a new fimbriae called CFA III was isolated and ch aracterized. In S.marcescens a gene for MR fimbria was cloned and expressed in E. coli. Its DNA sequence analysis showed that the sequence of amino acids at the N-terminal portion of the fimbrial subunit was almost the same as those of the other morphologically indistinguishable fimbria of E. coli. As a colonization factor for Vibrio cholerae the roles of hemagglution on the adherece on the intestinal mucosa of human was demonstrated.
The group of biolo
… More
gical activity of suface materiales: As one of the biological effects of the cell wall components, MDP,a muramyl dipeptide, exserted a stimulating effect against the toxic effect of the endotoxin. Mice pretreated with MDP showed an anaphylactic reaction when a small amount of LPS was administered intravacularly. The mechanisms of this phenomenon is remained to be elucidated. The role of coagulase and protein A on the infection of Staphylococcus aureus are investigate using the deficient mutant of these cell wall components. Coagulase is more closely related to the virulence of this bacteria.
The group of the invasiveness: For the analysis of the invasivenes of Shigella spp. the monoclonal antibodies for the cell surface of Sh. sonnei was produced. Some of the clones were reacted only with phase I strains of this bacteria. These antibodies might be usefull for the anlysis of invasive factors of this bacteria. The morphological and biological studies of the regular surface array ,an protective barrier on the cell surface, were carried out on Clostridium spp.. Less
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