| Project/Area Number |
60440029
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKAORI Shuji Kyoto University, Faculty of Medicine, Professor, 医学部, 教授 (10025538)
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| Co-Investigator(Kenkyū-buntansha) |
AKAIKE Akinori Kyoto University, Faculty of Medicine, Instructor, 医学部, 助手 (80135558)
FUJIWARA Motokazu Kyoto University, Faculty of Medicine,Instructor, 医学部, 助手 (60093308)
SASA Masashi Kyoto University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (20025654)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥23,600,000 (Direct Cost: ¥23,600,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1985: ¥19,500,000 (Direct Cost: ¥19,500,000)
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| Keywords | Depamine / Methamphetamine / Bromocriptine / Dopamine receptor / Nucleus accumbens / Caudate nucleus / Intracellular recording / イオントホレシス / ハロペリドール / ドンペリドン |
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| Research Abstract |
1. Electrophysiological study was performed to examine the actions of haloperidol and sulpiride on the nucleus accumbens neurons of rats receiving input form the parafascicular nucleus of thalamus, and to investigate whether or not the drugs antagonize the effects of exogenously applied dopamine and methamphetamine. The results indicated that exogenously applied dopamine as well as endogenously released dopamine produced an inhibition of the neuronal activities in the nucleus accumbens.
2. Methamphetamine also inhibited the nucleus accumbens neurons receiving input from the parafascicular nucleus of thalamus, probably by releasing dopamine form dopaminergic nerve terminals from the ventral tegmental area.
3. Microiontophoretic studies using anesthetized cats were performed to determine whether or not depamine D-1 and D-2 receptors co-exist in the same caudate nucleus neurons that receive inputs from the substantia nigra, and in which spikes elicited by nigral stimulation were blocked by domperidone, a selective D-2 antagonist. The results strongly suggested that the inhibitory D-1 and excitatory D-2 receptors co-exist on the same caudate neurons.
4. Effects of dopamine at low (1<mu>M) and high (100<mu>M) concentration on the rat caudate nucleus neurons were examined in a slice preparation using an intracellular recording technique. The results indicated that the postynaptic sites of caudate nucleus neurons have at least two subtype of dopamine receptors, D-1 and D-2 receptors, that mediate inhibitory and excitatory responses of the neuron to dopamine, respectively.
5. Effects of bromocriptine, a selective agonist for dopamine D-2 receptor, on the rat caudate nucleus neurons were examined in a slice preparation. The results implied that dopamine D-2 receptors activated by bromocriptine and a low concentration of dopamine mediated an excitation of the caudate nucleus neurons.
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