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Pathology of cells induced by paramyxovirus infection

Research Project

Project/Area Number 60440036
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionInstitute for Molecular and Cellular Biology, Osaka University (1985-1986, 1988)
Tokyo Institute of Technology (1987)

Principal Investigator

OKADA Y.  Professor Institute for Molecular and Cellular Biology, Osaka Univ., 細胞工学センター, 教授 (30029756)

末松 安晴 (1987)  東京工業大学, 工学部, 教授 (40016316)

Co-Investigator(Kenkyū-buntansha) SHIMA Yasufumi  Research Assistant Department of Fermentation techmnology, Facuty of Engineering, 工学部, 助手 (50187423)
浅田 雅洋  東京工業大学, 工学部, 助教授 (30167887)
荒井 滋久  東京工業大学, 工学部, 助教授 (30151137)
古屋 一仁  東京工業大学, 工学部, 助教授 (40092572)
金 在萬  京都薬科大学, 教授 (30029805)
石浦 正寛  国立基礎生物学研究所, 助手 (20132730)
Project Period (FY) 1985 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥18,900,000 (Direct Cost: ¥18,900,000)
Fiscal Year 1988: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1987: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1986: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1985: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordscDNA of F and HANA / fusion / naked lipid bilayer / cDNA / 限定分解 / 毒性 / NDV / cDNA注入 / 細胞融合 / 量子井戸構造 / 光スイッチ / 半導体レーザ / モノリシック光集積回路 / 多次元量子井戸構造 / 金属 / 絶縁体超格子構造 / FcDNA / HNcDNA / C蛋白質 / Fタンパク質 / HANAタンパク質 / ペプチドの人工合成 / HNタンパク質
Research Abstract

1. Mechanism of cell fusion by HVJ: On the basis of our experimental data, a hypothesis of the mechanism has been presented. After adsorption of HVJ onto cells, Fl N-terminal fusogenic domain inserted into cell membranes udergo conforma-tional change at 37゜C. This results in the folding in cholesterol molecule and the perturbation of the lipid bilayer, followed by marked damage of the cell mem-brane. This damage in turn induces disarragement of the cytoskeletal system, caused by the sudden introduction of Ca ions into the cytoplasm, and allows the membrane proteins to become freely mobile. The naked lipid bilayers formed by the clustering of IMP(Intramembrane particle) can then come into direct contact without interference from IMPs. In thee areas of attachment the cell membranes promptly fuse. Thus, the fundamental mechanism of cell fusion by HVJ seems to be similar to the fusion mechanism by PEG, electroporation and microsurgery of cell membranes.
2. This presentation is supported by … More the finding as follows: It has been demon-strated that aged HVJ virions easily fuse together by collision, but young ones immediately after the budding from infected cells show resistancy to this kind of fusion. It has been know that IMP are aggregated together in the envelope of aged virions, because of the disruption of the connection with M protein.
3. Simple liposomes can fuse with cells fusing together: It has been reported that liposomes containing diphtheria toxin fragment A show the activity of selective killing of HIV infected cells fusing with B-lymphocytes. It is possible to condiser that naked lipid bilayers are exposed on these cell surfaces and the liposomes become able to fuse with the areas.
4. cDNA of F and HANA glycoproteins have been isolated from HVJ or NDV infected cells. These cDNA plasmids containing SV40 ori showed high expressions when intro-duced in COS cells. Now expression of plasmids containing these cDNA and actin promoter are being observed in LLCMK2, CV1 and L cells. This project has not yet completed. Less

Report

(6 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report   Final Research Report Summary
  • 1986 Annual Research Report
  • 1985 Annual Research Report
  • Research Products

    (59 results)

All Other

All Publications (59 results)

  • [Publications] Y.Okada: Current Topics in Membrane and Transport. 32. 297-336 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Ikuta,S.Ueda,T.Uchida,Y.Okada,S.Kato: Jpn.J.Cancer Res.78. 1159-1163 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] J.Kim,Y.Okada: J.Memb.Biol.97. 241-249 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.Kaneda,T.Uchida,J.Kim,M.Ishiura,Y.Okada: Exp.Cell Res.173. 56-69 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.Kaneda,H.Hayes,T.Uchida,M.Yoshida,Y.Okada: Chromosoma(Berl). 96. 26-32 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 岡田善雄: 細胞工学. 6. 268-270 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.OKADA: "Sendai virus-mediated cell fusion" Current Topics in Membranes and Transport. 32. 297-336 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] J. KIM; Y. OKADA: "Difference in capacities for virion-to-virion fusion of young and aged HVJ(Sendai virus): A model of membrane fusion" J. Memb. Biol.97. 241-249 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.IKUTA; S.UEDA; T.UCHIDA; Y.OKADA; S.KATO: "Selective killing of human immunodeficiency virus-infected and-producing cells by liposomes containing diphtheria toxin fragment A" Jpn. J. Cancer Res.78. 1159-1163 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.KANEDA; T.UCHIDA; J.KIM; M.ISHIURA; Y.OKADA: "The improved efficient method for introducing macromolecules into cells using HVJ(Sendai virus) liposomes with gangliosides." Exp. Cell Res.173. 56-69 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] N.MIURA; E.OTSUKA; N.YAMABERI; M.IKEHARA; T.UCHIDA; Y.OKADA: "Use of the deoxyinosine-containing probe to isolate and sequence cDNA encoding the fusion(F) glycoprotein of Sendai virus(HVJ)." Gene. 38. 271-274 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.Okada: Current Topics in Membrane and Transport. 32. 297-336 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] K,Ikuta;S.Ueda;T.Uchida;Y.Okada;S.Kato: Jpn.J.Cancer Res.78. 1159-1163 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] J.Kim;Y.Okada: J.Memb.Biol.97. 241-249 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.Kaneda;T.Uchida;J.Kim;M,Ishiura;Y.Okada: Exp.Cell Res.173. 56-69 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.Kaneda;H.Hayes;T.Uchida;M.Yoshida;Y.Okada: Chromosoma(Berl). 96. 26-32 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 岡田善雄: 細胞工学. 6. 268-270 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] H.Yamamoto: Electron. Lett.VOL.21. 579-580 (1985)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Yamamoto: Trans. IECE of Japan. VOL.E68. 737-739 (1985)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Ohki: Jpn. J. Appl. Phys.VOL.24. L579-L581 (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.Kikugawa: Jpn. J. Appl. Phys.VOL.26. L1268-L1271 (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Yamamoto: IEEE J. of Lightwave Technol.(1988)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] J.Nayyer: IEEE J. of Lightwave Technol.(1988)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.G.Ravikumar: Electron. Lett.(1988)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.Asada: Jpn. J. Appl. Phys.VOL.24. L95-L97 (1985)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.Asada: IEEE J. Quantum. Electron.VOL.QE-22. 1915-1921 (1986)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Yang: Trans. IECE of Japan. VOL.E68. 521-523 (1985)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.Nagashima: Trans. IECE of Jpn.VOL.E68. 563-565 (1985)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Miyamoto: Trans. IECE of Jpn.VOL.E68. 796-797 (1985)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.Nagashima: Trans. IECE of Jpn.VOL.E-69. 92-94 (1986)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Miyamoto: Trans. IEICE of Jpn.VOL.E-70. 121-129 (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Miyamoto: Jpn. J. Appl. Phys.VOL.26. L176-L178 (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Miyamoto: Jpn. J. Appl. Phys.VOL.26. L225-L227 (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Miyamoto: Oyo Buturi. VOL.56. 357-360 (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Nakata: Electron. Lett.VOL.22. 58-59 (1986)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Nakata: Trans. IECE of Jpn.VOL.E69. 305-307 (1986)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Nakata: Surf. Sci.VOL.174. 519-521 (1986)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Nakata: IEEE J. of Quantum Electron.VOL.QE-22. 1880-1886 (1986)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Shimomura: Int. Symp. Technologies for Optoelectronics. (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Miyamoto: 2nd Biennal OMVPE. (1985)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Miyamoto: 13th European Conference on Optical Communication. 35 (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] P.Daste: 4th Conf. on MO-VPE. (1988)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Miyamoto: 4th Conf. on MO-VPE. (1988)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Nakata: 2nd Int. Conf. on Modulated Semiconductor Structures (MSS-II). (1985)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Nakata: 2nd International Conf.on Superlattices Microstructures and Microdevicers. (1986)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kim J.; Okada,Y.: J. Memb. Biol.97. 241-249 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ikuta,K.;Ueda,S.;Uchida,T.;Okada,Y.;Kato,S.: Jpn. J. Cancer Res. (Gann). 78. 1159-1163 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Okada,Y.: Current Topics in Membrane and Transport Academic Press Inc.32. 297-336 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 岡田善雄: 細胞工学. 16. 768-770 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 岡田善雄: J.Membrane Biology.

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡田善雄: Exp.Cell Res.

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡田善雄: Proc.Natl.Acad.Sci.U.S.A.83. 1476-1479 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡田善雄: Biochem.Biophys.Res.Commun.134. 1015-1021 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡田善雄: Proc.Natl.Acad.Sci.U.S.A.83. 4978-4982 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡田善雄: Chromosoma.

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡田善雄: "Cell Fusion By HVJ(Sendai Virus)." Academic Press, 30

    • Related Report
      1986 Annual Research Report
  • [Publications] 岡田善雄: "細胞融合と細胞工学" 中山書店, 66 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] GENE. 38. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] FEBS. 188-1. (1985)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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