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On the origin and adaptive changes of genetic restriction molecules on T cells

Research Project

Project/Area Number 60440037
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionUniversity of Tokyo

Principal Investigator

TADA Tomio  University of Tokyo, Professor, 医学部, 教授 (10009136)

Co-Investigator(Kenkyū-buntansha) YANAGI Yusuke  University of Tokyo, Assistant Professor, 医学部, 助手 (40182365)
ASANO Yoshihiro  University of Tokyo, Associate Professor, 医学部, 助教授 (70114353)
Project Period (FY) 1985 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥29,000,000 (Direct Cost: ¥29,000,000)
Fiscal Year 1988: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1987: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1986: ¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1985: ¥12,000,000 (Direct Cost: ¥12,000,000)
KeywordsT cell receptor / chain / I-J / Major histocompatibility complex / Restriction specificity / 組織適合遺伝子複合体 / 抑制T細胞 / 遺伝子再構成 / 遺伝的拘束 / 突然変異 / 遺伝子表現の排徐 / T細胞クローン / モノクローナル抗体 / 主要組織適合遺伝子複合体 / T細胞レセプター遺伝子
Research Abstract

T cells recognize antigen in context of major histocompatibility complex (MHC) class I or calss II antigens. In addition, it has been known that suppressor T cells (Ts) suppress the responses of other T cells with a genetic restriction imposed by the I-J antigen, whose genes have not been identified. Both above genetic restrictions are known to be adaptively acquired by T cells during their early ontogeny in the thymus under the influence of environmental MHC.
In order to elucidate the origin of MHC restrictions, we produced a large number of T cell clones from normal, F_1 and mice of radiation bone marrow chimeras. The cells were selected by the stimulation with antigen-presenting cells of different MHC haplotypes. The MHC restriction specificities of these clones with respect to antigen- and target cell interactions have been studied in parallel with the analysis of TcR and genes, and of expression of I-J molecules. It has been demonstrated that restriction-specificities of TcR and I-J on the same T cell clone are not necessarily identical, indicating that they are controlled by different genes. One of the T cell clones showed one and two genes rearranged in-frame, while only one gene was expressed on the cell surface. This clone had sequential changes in the restriction specificity suggesting that T cells may change restriction specificity after maturation. Finally we identified biochemically the I-J molecule in two dimensional gel as a molecular weight 86K dimer composed of two 43K glycopeptide subunits. The expression of this molecule in bone marrow chimeras was found to be adaptive to the thymic MHC.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1986 Annual Research Report
  • 1985 Annual Research Report
  • Research Products

    (74 results)

All Other

All Publications (74 results)

  • [Publications] Koyasu,Shigeo: Journal of Immunology. 134. 3130-3136 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Uracz,Wojciech: Proceedings of the National Academy of Sciences of the United States of America. 82. 2905-2909 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: Immunological Reviews. 83. 105-124 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Uracz,Wojciech: Nature. 316. 741-743 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yagi,Junji: European Journal of Immunology. 16. 497-503 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: Journal of Molecular and Cellular Immunology. 2. 232-233 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yanagi,Yusuke: European Journal of Immunology. 17. 1179-1182 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: BioEssays. 4. 283-285 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano,Yoshihiro: Journal of Immunology. 138. 667-673 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano,Yoshihiro: Journal of Experimental Medicine. 165. 87-96 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Iwashima,Makio: International Archives of Allergy and Applied Immunology. 83. 129-137 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Abe,Ryo: Journal of Immunology. 138. 2964-2969 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sano,Kunio: Journal of Experimental Medicine. 165. 1284-1295 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuji,Moriya: European Journal of Immunology. 17. 676-681 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano,Yoshihiro: Journal of Experimental Medicine. 166. 1613-1626 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama,Toshinori: Journal of Immunology. 139. 3935-3941 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano,Yoshihiro: Journal of Immunology. 140. 419-426 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama,Toshinori: European Journal of Immunology. 18. 761-765 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama,Toshinori: Immunology. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama,Toshinori: International Immunology. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "Immunopharmacology" Raven Press, 13-23 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "Monoclonal Antibody '84:Biological and Clinical Applications." Editrice Kurtis, 87-100 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "Idiotypes." Academic Press, 249-262 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "New Horizons in Animal Models for Autoimmune Disease." Academic Press, 341-347 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "H-2 Antigens." Plenum Press Corp., 435-440 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "The Semiotics of Cellular Communication in the Immune System." Springer-Verlag, 83-104 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "Cellular Basis of Immune Modulation." Alan R.Liss,Co., 341-351 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "Proceedings of the 19th International Symposium of the Princess Takamatsu Cancer Research Fund"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koyasu, shigeo: "Production of interleukin 3 and -interferon by an antigen-specific mouse suppressor T cell clone." Journal of Immunology.134. 3130-3136 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Uracz, Wojciech: "Involvement of I-J epitopes in the self- and allo-recognition sites of T cells: Blocking of self- and allo-MLR responders by monoclonal anti-I-J antibodies." Proceedings of the National Academy of Sciences of the United States of America. 82. 2905-2090 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: "I-J as an inducible T cell receptor for self." Immunological Reviews. 83. 105-124 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Uracz, Wojciech: "I-J epitopes are adaptively acquired by T cells differentiated in the chimeric condition." Nature. 316. 741-743 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yagi, Junji: "Ir gene expression on T cells: Effects of monoclonal antibodies directed against I region-controlled determinants on T cells." European Journal of Immunology. 16. 497-503 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: "The origin of MHC-restriction of suppressor T cells. A commentary." Journal of Molecular and Cellular Immunology. 2. 232-233 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yanagi, Yusuke: "Does the deletion within T cell receptor -chain gene of NZW mice contribute to autoimmunity in (NZB x NZW)F1 mice?" European Journal of Immunology. 17. 1179-1182 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: "Somatic generation of a genetic polymorphism: Towards the solution of the I-J enigma." BioEssays. 4. 283-285 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano, Yoshihiro: "Analysis of two distinct B cell activation pathways mediated by a monoclonal T helper cell. I. MHC-restricted activation of B cells by an IL-2 dependent pathway." Journal of Immunology. 138. 667-673 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano, Yoshihiro: "Epitopes associated with the MHC restriction site of T cells. II. Somatic generation of Iat epitopes on T cells in radiation bone marrow chimeras." Journal of Experimental Medicine. 165. 87-96 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Iwashima, Makio: "Alterations in the proliferative responses of T cells from aged and chimeric mice." International Archives of Allergy and Applied Immunology. 83. 129-137 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Abe, Ryo: "Epitopes associated with the MHC restriction site of T cells. I. selective expression of Iat epitopes on H-2-restricted heper T cells." Journal of Immunology. 138. 2964-29 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sano, Kunio: "MHC-restricted minimal regulatory circuit initiated by a class II-autoreactive T cell clone." Journal of Experimental Medicine. 165. 1284-1295 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuhi, Moriya: "Shared idiotopes between anti-class II monoclonal antibodies and major histocompatibility complex recognition site of T cells." European Journal of Immunology. 17. 676-681 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano, Yoshihiro: "Epitopes associated with MHC restriction site of T cells. III. I-J epitope on MHC-restricted T helper cells." Journal of Experimental Medicine. 166. 1613-1626 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama, Toshinori: "Major histocompatibility complex-restricted augmenting T cells induced by in vitro cultivation of antigen-primed T cells." Journal of Immunology. 139. 3935-3941 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano, Yoshihiro: "Analysis of two distinct B cell activation pathways mediated by a monoclonal T helper cell. II. T helper cell secretion of interleukin 4 selectively inhibits antigen-specific B cell activation by cognate, but not noncognate, interaction with T cells." Journal of Immunology. 140. 419-426 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama, Toshinori: "Epitopes associated with MHC restriction site of T cells. IV. I-J epitopes on MHC-restricted cloned T cells." European Journal of Immunology. 18. 761-765 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama, Toshinori: "I-J and mechanism of immunosuppression." Immunology. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama, Toshinori: "Biochemical identification of I-J as a novel dimeric surface molecule on mouse helper and suppressor T cell clones." International Immunology. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Furutani, Makoto: "Coexpression of two T-cell receptor -chain alleles in a single functional"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Utsunomiya, Naoko: "Unidirectional inhibition of early signal transduction of helper T cells by cloned suppressor T cells."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: Idiotypes. Academic Press, 249-262 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: Monoclonal Antibody '84: Biological and Clinical Application. Editrice Kurtis, 87-100 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: H-2 Antigens. Plenum Press Corp., 435-440 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: The Semiotics of Cellular Communication in the Immune System.Springer-Verlag, 83-104 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: Cellular Basis of Immune Modulation.Alan R. Liss,341-351 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Abe,R.: Journal of Immunology. 138. 2964-2969 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Asano,Y.: Journal of Experimental Medicine. 165. 87-96 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Asono,Y.: Journal of Experimental Medicine. 166. 1613-1626 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sano,K.: Journal of Experimental Medicine. 165. 1284-1295 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsuji,M.: European Journal of Immunology. 17. 675-681 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nakayama,T.: Journal of Immunology. 139. 3935-3941 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Asano,Y.: Journal of Immunology. 138. 667-673 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yagi,J.: Eur.J.Immunol.16. 497-503 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Asano,Y.: J.Exp.Med.165. 87-96 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Abe,R.: J.Immunool.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Sano,K.: J.Exp.Med.165(5). (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tsuji,M.: Eur.J.Immmunol.17. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Asano,Y.: J.Immunol.138. 667-673 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Okumura,K.: "Handbook of Experimental Immunology.Vol.2:Cellular Immunology.(Overview:Helper and suppressor T cells)" Blackwell Scientific Publications,Oxford, 71.1-71.7 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tada,T.: "Idiotypes.(The Immune circuit:somatic generation of epitopes involved in the mutual recognition by different subsets of T cells)" Academic Press, 2)408(249-26 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Journal of Immunology. 134-3130. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Proc.Natl.Acad.Sci.USA. 82-2905. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Nature. 316-741. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Immunological Reviews. 83-105. (1985)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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