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STUDIES OF IMMUNOLOGICAL PARTICIPATION OF THE SPLEEN IN ONSET OF IDIOPATHIC PORTAL HYPERTENSION

Research Project

Project/Area Number 60440061
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionOSAKA CITY UNIVERSITY

Principal Investigator

UMEYAMA Kaoru  OSAKA CITY UNIVERSITY MEDICAL SCHOOL, 医学部, 教授 (30046792)

Co-Investigator(Kenkyū-buntansha) FUZIMOTO Yasuhisa  OSAKA CITY UNIVERSITY MEDICAL SCHOOL, 医学部, 助手 (40173471)
YOSHIKAWA Kazuhiko  OSAKA CITY UNIVERSITY MEDICAL SCHOOL, 医学部, 助手 (50132204)
YAMASHITA Takafumi  OSAKA CITY UNIVERSITY MEDICAL SCHOOL, 医学部, 講師 (10094492)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 1987: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1986: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1985: ¥7,000,000 (Direct Cost: ¥7,000,000)
KeywordsIdiopathic portal hypertension / Lymphocyte subset / Overlap syndrome / Prolonged sensitization / アレルギー性臓器炎 / 肝再生抑制脾因子 / 免疫学的関与 / 遷延感作 / アレルギー性胆管炎
Research Abstract

1) Lymphocyte subset in peripheral blood and liver and spleen tissues was examined in patients with IPH. Leu 2a positive cell percent was low and 3a/2a ratio tended to be high in peripheral blood of the patients. On contrary, Leu 2a positive cell percent was high and 3a/2a ratio was low in the spleen. Immunohistochemical examination using monoclonal antibodies revealed that in follicles of the spleen, Leu 3a and Leu 7 positive cells were present in addition to B_1 positive cells in germinal center. A significant increase of Leu 4 positive cells in the marginal zone and Leu 2a positive cells in the red pulp were observed. These results indicated apparant abdominalities in lymphocyte subset in peripheral blood and the spleen.
2) Some cases with IPH had complication of other autoimmune diseases. There were three cases with AIHA, one PSS, one with SLE and one with Basedow's disease. They comprised about 6% of all patients. It was possible to consider them to be overlap syndrome. Twenty-nine … More cases been reported by now in Japan and this relationship is now under investigation in the cases.
3) To investigate the causes of IPH from the viewpoing of immunological mechanism, prolonged sensitization of rabbits was carried out with homologous and autologous spleen. Portal hypertention was observed in 47.9% of rabbits sensitized by homologous spleen. In animals sensitized by autologous spleen, hypertension was noted in 43.0%, and splenomegaly appeared with the increase of sensitization days. Examination of peripheral blood revealed marked pancytopenia in animals sensitized by homologous spleen. Autoantibody of antithyroid microsome antibody was detected in 62% of the animals. In the group sensitized by autologous spleen, leucocyte and platelet counts tended to decrease, and anti-DNA antibody appeared in some animals. These results are analogous to clinical findings of IPH, suggesting that immunological mechanism, allergic inflammation of organs in paticular, is involved occurence of IPH.
4) To study the intrasplenic factor depressing regeneration of the liver, incorporation of ^3H-thymidine into DNA fraction in DNA synthesis was examined in rat primary culture cells. DNA synthesis was markedly reduced when the splenic extract was added. The intrasplenic factor depressing liver regeneration was revealed to a protein with molecular weight of 50000-60000 dalton.This finding is thought to suggest that IPH is indication for splenectomy. Less

Report

(3 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • 1985 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 石川 哲郎,梅山 馨,山下 隆史,吉川 和彦: 医学のあゆみ. 135. 338-341 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 大平 雅一,谷浦 賢,鬼頭 秀樹,山下 隆史,梅山 馨: 医学のあゆみ. 136. 895-896 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 石川 哲郎,藤堂 泰三,吉川 和彦,橋本 仁,山下 隆史,梅山 馨: 消化器と免疫. 17. 417-420 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 梅山 馨,加藤 昇,坂口 茂,武田 過裕,石川 哲郎,藤本 泰久,吉川 和彦,山下 隆史: 厚生省特定疾患門脈血行異常症調査研究班昭和61年研究報告書. 75-80 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 梅山 馨,山下 隆史,石川 哲郎: 肝胆膵. 14. 889-897 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 加藤 昇,藤本 泰久,妙中 直之,坂口 茂,武田 温裕,山下 隆史,梅山 馨: 消化器と免疫. 21. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 梅山 馨,奥野 匡宥,山下 隆史: "消化器外科セミナー18.門脈圧亢進症における病態と治療-最近の進歩-" へるす出版, 252 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 梅山 馨,山下 隆史,吉川 和彦: "食道静脈瘤の治療" 医学教育出版, 152 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tetsuro Ishikawa, Kaoru Umeyama, Takafumi Yamashita, Kazuhiko Yoshikawa.: "Experimental model of idiopathic portal hypertension (IPH)-Prolonged sensitization in rabbits with human spleen of IPH." Journal of Clinical and Experimental Medicine. 135. 338-341 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tetsuro Ishikawa, Taizo Todo, Kazuhiko Yoshikawa, Hitoshi Hashimoto, Takafumi Yamashita, Kaoru Umeyama.: "Experimental idiopathic portal hypertension - Results of prolonged sensitization of rabbits with IPH and homologous spleen." Digestive Organ Immunology.17. 417-420 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masaichi Ohira, Kaoru Umeyama, Masaru Taniura, Takafumi Yamashita, Seiji Morisawa.: "An experimental study of a splenic inhibitory factor isfluencing hepatic regeneration." Surgery, Gynecology & Obsterics. 164. 438-444 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kaoru Umeyama, Noboru Kato, Shigeru Sakaguchi, Atsuhiro Takeda, Tetsuro Ishikawa, Yasuhisa Fujimoto, Kazuhiko Yoshikawa, Takafumi Yamashita.: "Investigation of the subsets of T-Lymphocytes in the spleen and liver with portal hypertension" 1986 Report of Ministry of Health and Walfare Research Committee on Abnormality of Portal Venous System.75-80 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kaoru Umeyama, Takafumi Yamashita, Tetsuro Ishikawa: "Lesion of the spleen in idiopathic portal hypertension." Kan.Tan.Sui.14. 889-897 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kaoru Umeyama, Takafuimi Yamashita, Kazuhiko Yoshikaw.: Treatment of esophageal varices. Igaku-kyoiku-shuppan, 26-36 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 梅山馨,石川哲郎,藤堂泰三,吉川和彦,橋本仁,山下隆史: 厚生省特定疾患門脈血行異常症調査研究班,昭和60年度研究報告書. 103-107 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 梅山馨,石川哲郎,藤堂泰三,吉川和彦,橋本仁,山下隆史: 厚生省特定疾患門脈血行異常症調査研究班,昭和60年度研究報告書. 108-110 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 石川哲郎: 日本外科学会雑誌. 87. 1461-1473 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 大平雅一: 日本外科学会雑誌. 87. 1548-1558 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 脾臓-基礎と臨床-. (1983)

    • Related Report
      1985 Annual Research Report
  • [Publications] 日本消化器病学会雑誌. 81-臨時増刊号. (1984)

    • Related Report
      1985 Annual Research Report
  • [Publications] 医学のあゆみ. 135-4. (1985)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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