| Project/Area Number |
60440064
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Nagoya City University |
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Principal Investigator |
MASAOKA Akira Nagoya City University, school of medicine,Professor, 医学部, 教授 (10028326)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAKAWA Yohsuke Nagoya City Univ.,school of medicine,Assistant, 医学部, 助手 (40148284)
SHIBATA Kazuo Nagoya City Univ.,school of medicine,Assistant, 医学部, 助手 (20145733)
HASHIMOTO Takahiko Nagoya City Univ.,school of medicine,Assistant Professor, 医学部, 助教授 (00080094)
YANAIHARA Noboru Shizuoka Preferectural Univ.,school of pharmacology,Professor, 薬学部, 教授 (80046250)
水野 武郎 名古屋市立大学, 医学部, 助教授 (50080156)
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| Project Period (FY) |
1985 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 1988: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1985: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | Serum thymic factor / Porcine serum / Radioimmunoassay / Thymus / Thymoma / Synthetic serum thymic factor / 放射免疫測定法 / STF / thymosin α_1 / 重症筋無力症 / Pro-STF / 胸腺ホルモン / 胸腺液性因子 / Serrm Thymic Factor(STE) / 合成STF, Thmosin α_1, 合成Thymosin / α_1, 抗STF抗血清 / 抗Thymosin α_1抗血清 / 胸腺腫培養上清 / 抗STF抗血清 / レクチン / 胸腺上皮細胞 / 肝 / 腎 / 免疫組織化学 |
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| Research Abstract |
The serum thymic factor(STF) is a nonapeptide initially isolated from porcine serm, but its true origin in the body has not yet been determined.
In an attempt to ascertain whether the presence of STF is restricted to the thymus, and to evaluate STF levels in the serum in various clinico-immunological conditions, we established a STF-specific radioimmunoassay as a substitute for the conventional indirect bioassay, and investigated the distribution and the chromatographic behavior of STF-like immunoreactivity in porcine plasma and various tissues, and in human plasma, thymus and thymoma.
The standard STF was synthesized according to its amino acid sequence, and C-terminal-specific anti-synthetic STF(R0116-5) was raised in rabbits against synthetic STF-BSA conjugate. The radioimmunoassay was sensitive to the synthe-tic STF in the range from 0.6pg to 640pg, and any cross-reactivity with other peptides was not detected.
In porcine plasma, the level of immunoreactive STF(IR-STF) was 32 5pg, whilst in healthy human plasma, the values were always below the minimal detectable level. The IR-STF was detected in the extracts of porcine thymus, liver, kindney and spleen, and levels in the liver and kidney were 2 to 3 times as high as in the thymus. In the human thymus and thymoma, with or without myasthenia gravis, levels were relatively high.
Gel filtration and HPLC reveled the presence of the larger molecular weight components in the IR-STF in all porcine and human tissues examined. Tripsinization of these components brought them to convert to a substance which corresponded closely in its elution on gel filtration and HPLC to that of syn-thetic STF.
Thus itis conceivable that STF is not a thymus-specific peptide, and the majo-rity of STF may exist in the thymus, thymoma,liver, spleen and kidney in a form of larger biosynthetic precursors for STF.
Comparison between STF and thymosin alpha-1 has been in progress.
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