Significance of bacterial pili on urinary tract infection and application of anti-pili antibody to the treatment
| Project/Area Number |
60440076
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Kyushu University |
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Principal Investigator |
KUMAZAWA Joichi Professor, Faculty of Medicine, Kyushu University, 医学部, 教授 (60038669)
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| Co-Investigator(Kenkyū-buntansha) |
OGATA Nobuo Assistant, Faculty of Medicine, Kyushu University, 医学部, 助手 (90185497)
MATSUMOTO Tetsuro Lecturer, Faculty of Medicine, Kyushu University, 医学部, 講師 (50150420)
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| Project Period (FY) |
1985 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 1988: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1986: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1985: ¥8,500,000 (Direct Cost: ¥8,500,000)
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| Keywords | Urinary tract infection / MR pili / MS pili / Superoxide / 多核白血球 / Type1線毛 / P線毛 / ディスムターゼ / タイプ1線毛 / 好中球機能 / 赤血球凝集反応 / 線毛 / 定着因子 / 抗線毛抗体 / 細菌線毛 / 大腸菌 |
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| Research Abstract |
1. Distribution of bacterial pili: Frequency of piliated bacteria in clinical isolates from urinary tract infeciton (UTI) was examined. High frequency was observed in E. coli and Serratia sp. and low frequency was observed in P. aeruginosa and Proteus sp. Mannose resistant (MR) and mannose sensitive (MS) pili was observed in E. coli and Serratia sp.
2. Cloning of pili-gene and host response of piliation: Cloned DNA was obtained from clinical isolate of S. marcescens and either MR or MS pili gene was transfered to non-piliated E.coli with gene manipulation technique. By the use of these new strain, MS piliated bacteria rather than MR pili stimulated more severely scar formation of the kidney. This scar formation was prophylacted by superoxide dismutase (SOD). This findings suggested that superoxide (SO) was produced by the stimulation of MS pili, and destructed surrounding renal tissue.
3. Host defense mechanism on UTI: Polymorphornuclear leukocytes (PMNL) was main factor of host defense mechanisms against UTI. However, function of PMNL was suppressed by ligh osmolality in the urine of renal medulla. As the main component of high osmolality in them was NaCl and urea, influence of high concentration of NaCl and ures on PMNL function was investigated. Energy dependent and indepent mechanisms were existed in it.
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Report
(5 results)
Research Products
(21 results)
