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Studies on the nature of tumor cell malignancy

Research Project

Project/Area Number 60440094
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionHokkaido University

Principal Investigator

KOBAYASHI Hiroshi  Hokkaido University, School of Medicine, Professor, 医学部, 教授 (20000911)

Co-Investigator(Kenkyū-buntansha) OIKAWA Tsuneyuki  Hokkaido University, School of Medicine, Instructor, 医学部, 助手 (80150241)
KUZUMAKI Noboru  Hokkaido University, School of Medicine, Professor, 医学部, 教授 (80091445)
TAKEICHI Noritoshi  Hokkaido University, School of Medicine, Associate Professor, 医学部, 助教授 (40002133)
HOSOKAWA Masuo  Hokkaido University, School of Medicine, Associate Professor, 医学部, 助教授 (20001901)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥29,500,000 (Direct Cost: ¥29,500,000)
Fiscal Year 1986: ¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1985: ¥23,000,000 (Direct Cost: ¥23,000,000)
KeywordsTumor cells / Malignancy / Tumor progression / Tumor regression / Tumor metastasis / Cell surface modification / Oncogene / 細胞間コミュニケーション / 腫瘍原性 / 転移能 / 突然変異 / DNAメチル化阻害 / 細胞融合 / ウイルス感染
Research Abstract

Tumor cells are composed of heterogeneous populations. The grade of tumor cell malignancy, therefore, varies from cell to cell, and certain populations of tumor cells are highly malignant when their tumorigenicity and ability to metastasize are examined, while other are not.
In this study, we attempted to investigate the factors by which tumor cells are led to become more malignant during their in vivo proliferation. In order to achieve the purpose, we selected mutagenic chemicals (quercetin, EMS and MNNG), viruses, a DNA-hypomethylating agent (5-azacystidine), anti-differentiation agents (TPA, indomethacin) and the hybridization technique for the modification of in vivo and in vitro tumorigenicity of tumor cells. We also examined natures of tumor cells such as the oncogene expression and the ability of tumor cells to communicate to normal cells and to other tumor cells in the view points of the correlation to their metastasizing ability.
As the result, we found that the tumor cell malignancy could be altered by some of the above means, and noted that an oncogene expression (c-fos) well correlated to their metastasizing ability and that the grade of communication of tumor cells to normal fibroblasts showed a reverse correlation to their metastasizing ability.

Report

(2 results)
  • 1986 Final Research Report Summary
  • 1985 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Kobayashi,H.: J.Biol.Response Mod.5. 1-11 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okayasu,T.: Jpn.J.Cancer Res.(Gann). 77. 181-185 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yuhki,N.: Jpn.J.Cancer Res.(Gann). 77. 9-12 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kuzumaki,N.: J.Natl.Cancer Inst.77. 1273-1279 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Oikawa,T.: Int.J.Cancer. 39. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Morikawa,K.: Cancer Research. 46. 684-688 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kobayashi,H.: "Modification of the tumor cell:regression and progression of tumor cells by the administration of viruses and chemicals." International Congress Series No.738,Excerpta Medica, 336 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Fujii,Y.: "Host Defense Mechanisms Against Cancer" International Congress Series No.738,Excerpta Medica, 336 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kobayashi, H.: "The biological modification of tumor cells as a means of inducing their regression: an overview." J. Biol. response Mod.5. 1-11 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okayasu, T.: "The active immunotherapy of a 3-methylcholanthrene-induced rat tumor with Friend virus-infected (xenogenized) tumor cells." Jpn. J. Cancer Res. (Gann). 77. 181-185 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yuhki, N.: "Metastatic ability and expression of c-fos oncogene in cell clones of a spontaneous rat mammary tumor." Jpn. J. Cancer Res. (Gann). 77. 9-12 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kuzumaki, N.: "Establishment of four mouse hybridoma cell lines producing monoclonal antibodies reactive with ras oncogene product p21." J. Natl. Cancer Inst.77. 1273-1279 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Oikawa, T.: "Suppression of transformed phenotypes in intraspecific somatic cell hybrid clones between the c-myc activation mouse plasmacytoma." Int. J. Cancer. 39. in press (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kobayashi, H.: International Congress Series No. 738, Excerpta Medica. Modification of the tumor cell: regression and progression of tumor cells by the administration of viruses and chemicals., 277-295 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Jpn.J.Cancer Res.(Gann). 77-1. (1986)

    • Related Report
      1985 Annual Research Report
  • [Publications] Neoplasma. 32-4. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Jpn.J.Cancer Res.(Gann). 76-1. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Cancer Res.45-2. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Cancer Res.45-4. (1985)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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