Paramagnetic Resonance Absorption of cytochrome c in the frequency Range 20-50 MHz.

Research Project

Project/Area Number	60460028
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	固体物性
Research Institution	SAITAMA UNIVERSITY
Principal Investigator	ONO Kazuo SAITAMA UNIVERSITY, 理学部, 教授 (80013449)
Co-Investigator(Kenkyū-buntansha)	KIMURA Keisaku INSTITUTE FOR MOLECULAR SCIENCE, 助手 (70106160) ISHIWATA Mitsumasa SAITAMA UNIVERSITY, 理学部, 助手 (80184571) MOTOYA Kiyoichiro SAITAMA UNIVERSITY, 理学部, 助教授 (60114683)
Project Period (FY)	1985 – 1986
Project Status	Completed (Fiscal Year 1986)
Budget Amount *help	¥7,500,000 (Direct Cost: ¥7,500,000) Fiscal Year 1986: ¥1,700,000 (Direct Cost: ¥1,700,000) Fiscal Year 1985: ¥5,800,000 (Direct Cost: ¥5,800,000)
Keywords	Paramagnetic resonance absorption / 長波長電子スピン共鳴吸收
Research Abstract	It was reported by the authers that the electronic state of <Fe^(3+)> of the oxidized cytochrome <c_3> is a low spin state and the heme-heme spin interaction was observed to be more than about ten times than expected. The interaction consists of two components; the dipolar interaction, <W_(> alpha <:p)> and the exchange interaction, Wex. The results obtained by us suggested that Wex>> <W_(> alpha <:p)> . In ordef to investigate the interaction directly, the frequency dependence of the absorption shape in the paramagnetic resonance has been measured in the microwave frequency range between 20 and 50 MHz, where <mu> Hres may grow up into the interaction energy as the frequency is increased. We have tried to observe the resonance absorption at temperatures down to 1.4 K, but we have not clearly observe any absorption. Considering the sensitivity of our ESR apparatus, the absorption line width may be estimated as several tens Oersteds which may come from the dipolar interaction. Therefore, on the contrary of our expectation, we may conclude that Wex<< <W_(> alpha <:p)> .