| Project/Area Number |
60460218
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Toyama Medical & Pharmaceutical University |
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Principal Investigator |
UENO Masaharu Department of Hospital Pharmacy, Toyama Medical & Pharmaceutical University, Associate Professor, 付属病院, 助教授 (40080197)
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| Co-Investigator(Kenkyū-buntansha) |
ADACHI Isao Department of Hospital Pharmacy, Toyama Medical & Pharmaceutical University, Res, 付属病院, 助手 (30151070)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1985: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Liposome / Detergent / Mixed Micelle / Diameter / Transient State / Time Factor / アンバライトXAD-2 / リポソーム / 準弾性光散乱 / 流体力学的半径 / フリーズフラクチャー / 粒子径 |
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| Research Abstract |
Phospholipid vesicles, or liposomes were prepared by detergent removel using hydrophobic porous beads, Amberlite XAD-2, or dialysis from detergent-phospholipid mixed micelles. The liposomes formed were found to be mostly unilammellar vesicles. The liposome size was dependent on the methods of detergent removal and a type of detergents. Changes in forms of phospolipid-detergent mixture aggregates with decreasing detergent content-were pursuited by quasi elastic light scattering, gel-exclusion chromatography on Sephacryl S-1000, electron microscopy, and turbidity of the suspension. On the basis of the data, a possible model of the mechanism for transformation of the mixed micelle to liposome was proposed. The model is characterized by a chain-like aggregation entering an intermediate state before liposome formation. The liposome size and its distribution are dominated by the time factor in passing the transient state.
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