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Biosynthesis of chlorophyl studied by time-resolved Raman spectroscopy

Research Project

Project/Area Number 60460239
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionFaculty of Science, University of Tokyo

Principal Investigator

KOBAYASHI Takayoshi  Faculty of Science, University of Tokyo, 理学部, 助教授 (60087509)

Co-Investigator(Kenkyū-buntansha) UCHIKI Hisao  Faculty of Scinece, University of Tokyo, 理学部, 助手 (50142237)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1985: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsPicosecond / Time-resolved / Raman spectroscopy / Chlorophyl / ピコ秒 / クロロフィル生合成 / 時間分解ラマン分光 / プロトクロロフィリド・ホロクローム / ラマンスペクトル / 光反応中間体 / 黄化葉
Research Abstract

There were two targets of this project: (1)By detailed study of picosecond to microsecond time-resolved absorption spectroscopy, new intermediates in the biosynthetic processes of chlorophyl in the time region were to be discovered and from the dynamical study, the time constants of the processes were to be determined. (2)From the time-resolved resonance Raman spectra of the intermediates, the structure were expected to be determined.
Concerning the first target, after improving the apparatus, and repeating the experiment the biosynthetic process was found to be described as:
PChlide-650->PChlide(S_1)->^^^< PS>X-B ->^^^<<plus-minus>0.2<micrn>S>X-690 ->^^^<<plus-minus>20<micrn>S>X-684 ->^^^<<plus-minus>0.5<micrn>S> Chlide-678->^^^<5<plus-minus>2.0<micrn>S>Chla.
All the intermesiates were found to be correspondent with those at low temperature.
Concerning the second targer, it was very difficult to observe the timeresolved resonance Raman spectra of the newly found and other intermediates because of the very in tense fluorescence. At the present stage, we have not yet succeeded in getting the Raman spectra. However, the apparatus constructed for the time-resolved Raman spectra was used for the determination of femtosecond vibrational phase relaxation by observing CARS or CSRS using broad band dye laser on the basis of incoherent light method. The work was extendod to the electronic dephasing time measurement and these works were highly evaluated by many scientific research people. The head investigator was invited more than six times to give talks at the international conferences and was asked to write more than three invited papers.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] S. Koshihara;T. Kobayashi: J. chem. Phys.85. 1211-1219 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Ohtani;T. Kobayashi;M. Tuda: Photobiochemistry and Photobiophysics. 13. 203-208 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K. Kurokawa;T. Hattori;T. Kobayashi: Physical Review A. 36. 1298-1304 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Koshihara and T.Kobayashi: "Time-resolved resonance Raman spectrum of chrysene in the S and T state" J.Chem.Phys.85. 1211-1219 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Ohtani, T.Kobayashi, and M.Tuda: "Photocycle of sensoty rhodopsin:a new precursot of sR370" Photobiochemistry and photobiophysics. 13. 203-208 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Kurokawa, T.Hattori, and T.Kobayashi: "Subpicosecond molecular dynamics studied by degenerate four-wave mixing with incoherent light" Physical Review A. 36. 1298-1304 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shinya Kashihara;Takayoshi Kobayashi: Journal of Chemical Physics. 85. 1211-1219 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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