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STUDY ON MULTI-FED-BATCH CULTURE

Research Project

Project/Area Number 60470120
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 発酵工学
Research InstitutionNAGOYA UNIVERSITY

Principal Investigator

SHIMIZU SHOICHI  PROF. DEPT. OF FOOD SCI. & TECHNOL., SCHOOL OF AGRIC., NAGOYA UNIV., 農学部, 教授 (00025842)

Co-Investigator(Kenkyū-buntansha) YAMANE TSUNEO  ASSOC.PROF. DEPT. OF FOOD SCI. & TECHNOL., SCHOOL OF AGRIC., NAGOYA UNIV., 農学部, 助教授 (70026102)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1987: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1985: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsmulti-fed-batch culture / methylotroph / Streptomyces laurentii / Pseudomonas fluorescens / automatic feeding / high cell mass cultivation / thiostrepton / Iaurentii / 高菌帯濃度流加培養法のコスト / リパーゼ / 排ガスのC【O_2】濃度 / 原価計算式
Research Abstract

A fed-batch culture where more kinds of nutrient than two are supplied simultaneously is defined as multi-fed-batch culture and theoretical and experimental studies were performed to develop optimization and automation techniques of the multi-substrate feedings. In the multi-fed- batch culture, the fed-substrates are either multiple compounds or natural nutrients (such as corn steep liquor, defatted soy been meal or yeast extract). As the former case, production of serine from glycine was attempted with a methylotroph by supplying both methanol and oxygen gas automatically. Both methanol concentration and dissolved oxygen concentration were kept constant by detector-controller systems, by which a high final concentration fo serine was accomplished.
Since no direct detector system is available for the case of natural nutrients, one has to resort to indirect control indices to feed them automatically. Possible indirect indices are pH and CO2 concentration in the exhaust gas. Effective production of thiostrepton by Streptomyces laurentii was achieved with use of pH-stat modal feeding of multi-substrates. Mass production of lipase by Pseudomonas fluorescens was possible with automatic feeding of olive oil whose feed rate was proportional to the CO_2 evolution ate.
Economic significance of high cell mass cultivation was evaluated theoretically in terms of both raw material cost and running cost.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Takahiro SUZUKI: Biotechnology and Bioengineering. 192-201. 1985

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: Journal of Fermentation Technology. 64. 63-70 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: Jounal of Fermentation Technology. 64. 317-326 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Pramote SIRIROTE: Jounal of Fermentation Technology. 64. 389-396 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: Applied Microbiology and Biotechnology. 25. 526-531 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: Applied Microbiology and Biotechnology. 28. 417-422 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: Applied Microbiology and Biotechnology. 28. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: Jounal of Fermentation Technology. 66. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tsuneo YAMANE: Journal of Fermentation Technology. 66. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 清水祥一: "バイオリアクターシステム" 共立出版株式会社, 120 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: "Automatic supplementation of minerals in fed-batch culture to high cell mass concentration" Biotechnology and Bioengineering. 27. 192-201 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: "Detection and automatic control of ammonium concentration in microbial culture with an ammonium ion selective electrode" Journal of Fermentation Technology. 64. 63-70 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: "Control ofcarbon source supply and dissolved wxygen by use of carbon dioxide concentration of exhaust gas in fed-batch culture" Journal of Fermentation Technology. 64. 317-326 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Pramote SIRIROTE: "Production of L-serine from methanol and glycine by resting cells of a methylotroph under automatically controlled conditions" Journal of Fermentation Technology. 64. 389-396 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: "Mass production of thiostrepton by fed-batch culture of Streptomyces laurentii with pH-stat modal feeding of multi-substrate" Applied Microbiology and Biotechnology. 25. 526-531 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: "Mass production of lipase by fed-batch culture of Pseudomonas fluorescens" Applied Microbiology and Biotechnology. 28. 417-422 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: "Effect of ammonium feeding on production of thiostrepton by fed-batch culture" Applied Microbiology and Biotechnology. 28. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: "Effect of control of glucose feeding on bacitracin production by fed-batch culture of Bacillus licheniformis" Journal of Fermentation Technology. 66. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tsuneo YAMANE: "Economic significance of increasing cell mass concentration for metabolite production - A theoretical study -" Journal of Fermentation Technology. 66. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shoichi SHIMIZU: Kyoritsu Publishing Company, Limited. Bioreactor System, 120 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro SUZUKI: Applied Microbiology and Biotechnology. 23. 322-329 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Takahiro SUZUKI: Jounal of Fermentation Technology. 64. 63-70 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Takahiro SUZUKI: Applied Microbiology and Biotechnology. 23. 366-369 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Takahiro SUZUKI: Applied Microbiology and Biotechnology. 23. 370-374 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Takahiro SUZUKI: Journal of Fermentation Technology. 64. 317-326 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Pramote SIRIROTE: Jounal of Fermentation Technology. 64. 389-396 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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