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Studies on the specific biosynthesis and degradation of complex carbohydrates by use of microbes and their application

Research Project

Project/Area Number 60470131
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 発酵・醸造
Research InstitutionKyoto University

Principal Investigator

TOCHIKURA Tatsurokuro  Kyoto University, 農学部, 教授 (70026524)

Co-Investigator(Kenkyū-buntansha) YANO Toshihiro  Kyoto University, 農学部, 教務職員 (30135553)
YAMAMOTO Kenji  Kyoto University, 農学部, 助手 (70109049)
TACHIKI Takashi  Kyoto University, 農学部, 助手 (60026573)
KUMAGAI Hidehiko  Kyoto University, 農学部, 助教授 (70027192)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1985: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsBaker's yeast fermentation / Coupling with energy tranfer / Sugar nucleotides / Acetyl-hexosaminidases / Fucosidase / Epimerase / 複合糖質糖鎖
Research Abstract

[Objective] 1. To investigate production of sugar nucleotides by combinations of fermentation and enzymatic methods, and reserve supply system of yeast fermentation energy.
2. To investigate enzymatic preparations of UDP-N-acetylgalactosamine and GDP-fucose and their application to specific biosynthesis of complex carbohydrates.
3. To investigate production of mold <beta> -N-acetylhexosaminidase and <alpha> -L-fucosidase and their characterization.
4. To investigate chemical structure and biological role of complex carbohydrates by use of mold glycosidases.
[Approach and Results]
1. Production of UDP-N-acetylgalactosamine and GDP-mannose by fermentation with energy transfer; efficiency of ATP energy in coupled fermentation.
2. Preparation of UDP-N-acetylgalactosamine from UDP-N-acetylglucosamine by Bacillus subtilis epimerase; application of UDP-GalNAc as specific reagent for identifying rare blood group; purification and characterization of Bacillus UDP-GlcNAc 4-epimerase.
3. Production and characterization of glucosidases which liberate monosaccharide units from sugar chains of glycoconjugates; characterization of <beta> -N-acetylhexosaminidase with higher activity of GalNAcase than that of GlcNAcase from Penicillium oxalicum; effective biosynthesis and purification of Fusarium oxysporum <alpha> -L-fucosidase.
4. Elucidation of structure of sugar chains of complex carbohydrates.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Kenji YAMAMOTO: Agricultural and Biological Chemistry. 49. 603-609 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kenji YAMAMOTO: Agricultural and Biological Chemistry. 49. 611-619 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kenji YAMAMOTO: Agricultural and Biological Chemistry. 49. 2463-2464 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Toshihiro YANO: Agricultural and Biological Chemistry. 49. 3179-3187 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kenji YAMAMOTO: Applied and Environmental Microbiology. 50. 1019-1023 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kenji YAMAMOTO: Agricultural and Biological Chemistry. 50. 1689-1695 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kenji YAMAMOTO: "Purification and Some Properties of UDP-N-Acetylglucosamine 4-Epimerase from Bacillus subtilis" Agricultural and Biological Chemistry. 49. 603-609 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kenji YAMAMOTO: "Purification and Characterization of <beta> -N-Acetylhexosaminidase from Penicillium oxalicum" Agricultural and Biological Chemistry. 49. 611-619 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Toshihiro YANO: "Purification and Characterization of a Novel <alpha> -L-Fucosidase from Fusarium oxysporum Grown on Sludge" Agricultural and Biological Chemistry. 49. 3179-3187 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kenji YAMAMOTO: "Purification and Properties of <beta> -N-Acetylhexosaminidase from Mucor fragilis Grown in Bovine Blood" Applied and Environmental Microbiology. 50. 1019-1023 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kenji YAMAMOTO: "Induction and Purification of <alpha> -L-Fucosidase from Fusarium oxysporum" Agricultural and Biological Chemistry. 50. 1689-1695 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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