| Project/Area Number |
60470131
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
発酵・醸造
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YANO Toshihiro Kyoto University, 農学部, 教務職員 (30135553)
YAMAMOTO Kenji Kyoto University, 農学部, 助手 (70109049)
TACHIKI Takashi Kyoto University, 農学部, 助手 (60026573)
KUMAGAI Hidehiko Kyoto University, 農学部, 助教授 (70027192)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1985: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | Baker's yeast fermentation / Coupling with energy tranfer / Sugar nucleotides / Acetyl-hexosaminidases / Fucosidase / Epimerase / 複合糖質糖鎖 |
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| Research Abstract |
[Objective] 1. To investigate production of sugar nucleotides by combinations of fermentation and enzymatic methods, and reserve supply system of yeast fermentation energy.
2. To investigate enzymatic preparations of UDP-N-acetylgalactosamine and GDP-fucose and their application to specific biosynthesis of complex carbohydrates.
3. To investigate production of mold <beta> -N-acetylhexosaminidase and <alpha> -L-fucosidase and their characterization.
4. To investigate chemical structure and biological role of complex carbohydrates by use of mold glycosidases.
[Approach and Results]
1. Production of UDP-N-acetylgalactosamine and GDP-mannose by fermentation with energy transfer; efficiency of ATP energy in coupled fermentation.
2. Preparation of UDP-N-acetylgalactosamine from UDP-N-acetylglucosamine by Bacillus subtilis epimerase; application of UDP-GalNAc as specific reagent for identifying rare blood group; purification and characterization of Bacillus UDP-GlcNAc 4-epimerase.
3. Production and characterization of glucosidases which liberate monosaccharide units from sugar chains of glycoconjugates; characterization of <beta> -N-acetylhexosaminidase with higher activity of GalNAcase than that of GlcNAcase from Penicillium oxalicum; effective biosynthesis and purification of Fusarium oxysporum <alpha> -L-fucosidase.
4. Elucidation of structure of sugar chains of complex carbohydrates.
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