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The structure of aldolase isozyme genes and the regulation of their tissue-specific expression

Research Project

Project/Area Number 60470142
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionYamagata University

Principal Investigator

KIICHI Ishikawa  Yamagata University School of Medicine, 医学部, 教授 (40018312)

Co-Investigator(Kenkyū-buntansha) REIKO Tsutsumi  Yamagata University School of Medicine, 医学部, 助手 (20005664)
KEN-ICHI Tsutsumi  Yamagata University School of Medicine, 医学部, 助手 (40113964)
SOH Hidaka  Yamagata University School of Medicine, 医学部, 助手 (00142224)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1985: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsAldolase B gene / Regulation of gene expression / DNase I hyper sensitive region / DNAメチレーション
Research Abstract

By examining the tissues and cells which express or do not express aldolase B gene, the relationship between the gene expression and chromatin structure and DNA methylation of aldolase B gene was studied. Following results were obtained. (1) The examination of the chromatin structure of aldolase B locus by DNase I hypersensitivity revealed that the specific structure which is sensitive to DNase I at about 0.3 Kb upstream from the transcription-initiation site is necessary for transcription of the gene. (2) Out of 15 Hpa <II> and Hha <I> sites in and around the gene, those sites which located at 3'-half and at downstream of the gene were heavily methylated, regardless of the gene expression. A Hha <I> site in the 5'-flanking region, and several Hpa <II> sites in the first intron were hypomethylated in liver and differentiated hepatoma, while they were heavily methylated in brain and undifferentiated hepatoma. These facts suggest that the hypomethylation of cytosine residue at specific sites in the 5'-flanking and the 5'-half regions of the gene are related to the expression of this gene. Our recent results suggest that, besides loosened chromatin structure and hypomethylation, some trans-acting factor(s) which interact with the element in this region are necessary for the transcription of this gene.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] K.Tsutsumi: J.Mol.Biol.181. 153-160 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 石川喜一: 蛋白質 核酸 酵素. 31. 201-205 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.Daimon: J.Biochem.100. 1279-1286 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] R.Tsutsumi: Biochem.Int.13. 781-786 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] J.Sato: Arch.Biochem.Biophys.253. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] K.Tsutsumi: J.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] K.Tsutsumi: "Tissue-specific and developmentally specific controls involved in rat aldolase B gene expression in Isozymes:Current topics in biological and medical research(C.L.Markert ed.)" Alan R.Liss Inc.,New York, 18 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] K.Tsutsumi; T.Mukai; S.Hidaka; H.Miyahara; R.Tsutsumi; T.Tanaka; K.Hori; K.Ishikawa: "Structure and genomic organization of the rat aldolase B gene" Journal of Molecular Biology. 181. 153-160 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.Daimon; K.Tsutsumi; K.Ishikawa: "DNA methylation and the regulation of aldolase B gene expression" Biochemistry International. 100. 1279-1286 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] R.Tsutsumi; K.Isutsumi; K.Ishikawa: "In vivo processing of rat aldolase B mRNA precursor" Biochemistry International. 13. 781-786 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] J.Sato; K.Tsutsumi; M.Ishikawa; K.Ishikawa: "Dietary regulation of aldolase isozyme expression in rat intestinal mucosa" Archives of Biochemistry and Biophysics. 253. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] K.Tsutsumi; R.Tsutsumi; K.Ishikawa: "Tissue-specific chromatin structure of the rat aldolase B locus" Journal of Biochemistry.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Isozymes: Current topics in biological and medical research (C.L.Markert ed.) K.Tsutsumi, R.Tsutsumi, M.Daimon, M.Numazaki, K.Ishikawa: Alan R. Liss Inc., New York. Tissue-specific and developmentally specific controls involved in rat aldolase B gene expression, (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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