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Control of Solubilized State and Estimation of Molecular Organization of Membrane Proteins by the Use of Analogues of Sodium Dodecyl Sulfate

Research Project

Project/Area Number 60470157
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionInstitute for Protein Research

Principal Investigator

TAKAGI Toshio  Institute for Protein Research,Osaka University, 蛋白質研究所, 教授 (00029943)

Co-Investigator(Kenkyū-buntansha) HAYASHI Yutaro  School of Medicine,Kyorin University, 医学部, 講師 (10086556)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1985: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywords電気泳動 / 生体膜蛋白質 / 可溶化 / 界面活性剤 / 電気詠動
Research Abstract

In the field of biochemical research,an anionic surfactant,sodium dodecyl sulfate(SDS),is routinely used.We have focused our attention on the potentiality of analogues of SDS of which general structure can be described as follows:n-C_<12>H_<25>(OCH_2CH_2)OSO^<3->X^+.It was found that:
(1)among the analogues having their cations replaced by Li,Tris,triethanolammine and triisopropanolammine,the first was stronger even than SDS as protein denaturant,and the others were far less destructive than SDS as protein denaturant,and the others were far less destructive than SDSbecoming milder on the sequence mentioned above;
(2)the series of surfactant could be used to determine the hierarchy of organization of molecular assemblies of proteins especially membrane proteins;
(3)a typical example of the successful application of the series is the separation of the products of disassembly of thylakoid chlorophyllproteins in which different surfactant of the series giving different electrophoretic patterns;
(4)analogues with m=2to8 were compared with to reveal that only those with m equal to or larger than 6 have high affinity to membrane proteins and thus allows efficient separation of mambrane prteins in the presence of such a surfactant;
(5)a readily available surfactant similar to those described above with distribution with respect to both alkyl chain length (number of carbon atoms around 13) and oxyethylene chain length(around m =8)was found to be an efficient additive in polyacrylamide gel elctrophoresis for separation of the subunits of (Na,K)-ATPase without causing denaturation.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kanenobu Kubo;Toshio Takagi: Collagen Rel. Res.4. 201-208 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanenobu Kubo;Tohsio Takagi: J. Biochem.99. 1545-1548 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanenobu Kubo;Tohsio Takagi: Anal. Biochem. 156. 11-16 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 高木俊夫: 油化学.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanenobu Kubo,Nobuo Okabe,Toshio Takagi: "Separation of Cross-linked Products of Rat Tail Tendon Collagen from Monomeric Components by %gel Chromatogtaphy in the Presence of Tris Dodecyl Sulfate at 4゜C" Collagen Rel. Res.5. 149-156 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanenobu Kubo,Toshio Takagi: "Polyacrylamide Gel Electrophoresis in the Presence of Lithium Dodecyl Sulfate -Problems and Merits-" Seikagaku. 57. 117-120 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanenobu Kubo,Toshio Takagi: "Marked Effect of Cations on the Separation of Spinach Thylacoid Chlorophyll-proteins in Polyacrylamide Gel Electrophoresis in the Presence of Alkanolammonium Dodecyl Sulfates" J.Biochem.99. 1545-1548 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanenobu Kubo,Toshio Takagi: "Binding of Lithium Dodecyl Sulfate to Polyacrylamide Gel at 4゜C Perturds Electrophoresis of Proteins" Anal.Biochem.156. 11-16 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Misao Koide,Masahiro Fukuda,Kazuo Ohbu,Yasushi Watanabe,Yutaro Hayashi, Toshio Takagi: "Polyacrylamide Gel Electrophoresis of Several Proteins in the Presence of Sodium Oligooxyethylene Dodecyl Ether Sulfates or a Commercially Available Analog" Anal. Biochem.164. 150-155 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanenobu Kubo;Toshio Takagi: Collagen Rel.Res.5. (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] Kanenobu Kubo;Toshio Takagi: J.Biochem.99. 1545-1548 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Kanenobu Kubo;Toshio Takagi: Anal.Biochem.156. 11-16 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Shio Makino;Shigenori Maezawa;Ryuichi Moriyama;Toshio Takagi: Biochem.Biophys.Acta. 874. 216-219 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Misao Koide;Masahiro Fukuda;Kazuo Ohbu;Yasushi Watanabe;Yutaro Hayashi;Toshio Takagi: Anal.Biochem.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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