Control of Solubilized State and Estimation of Molecular Organization of Membrane Proteins by the Use of Analogues of Sodium Dodecyl Sulfate
| Project/Area Number | 60470157 |
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Institute for Protein Research |
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Principal Investigator |
TAKAGI Toshio Institute for Protein Research,Osaka University, 蛋白質研究所, 教授 (00029943)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Yutaro School of Medicine,Kyorin University, 医学部, 講師 (10086556)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed(Fiscal Year 1987)
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| Budget Amount *help |
¥4,900,000 (Direct Cost : ¥4,900,000)
Fiscal Year 1987 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1986 : ¥1,700,000 (Direct Cost : ¥1,700,000)
Fiscal Year 1985 : ¥2,700,000 (Direct Cost : ¥2,700,000)
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| Keywords | 電気泳動 / 生体膜蛋白質 / 可溶化 / 界面活性剤 / 電気詠動 |
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| Research Abstract |
In the field of biochemical research,an anionic surfactant,sodium dodecyl sulfate(SDS),is routinely used.We have focused our attention on the potentiality of analogues of SDS of which general structure can be described as follows:n-C_<12>H_<25>(OCH_2CH_2)OSO^<3->X^+.It was found that:
(1)among the analogues having their cations replaced by Li,Tris,triethanolammine and triisopropanolammine,the first was stronger even than SDS as protein denaturant,and the others were far less destructive than SDS as protein denaturant,and the others were far less destructive than SDSbecoming milder on the sequence mentioned above;
(2)the series of surfactant could be used to determine the hierarchy of organization of molecular assemblies of proteins especially membrane proteins;
(3)a typical example of the successful application of the series is the separation of the products of disassembly of thylakoid chlorophyllproteins in which different surfactant of the series giving different electrophoretic patterns;
(4)analogues with m=2to8 were compared with to reveal that only those with m equal to or larger than 6 have high affinity to membrane proteins and thus allows efficient separation of mambrane prteins in the presence of such a surfactant;
(5)a readily available surfactant similar to those described above with distribution with respect to both alkyl chain length (number of carbon atoms around 13) and oxyethylene chain length(around m =8)was found to be an efficient additive in polyacrylamide gel elctrophoresis for separation of the subunits of (Na,K)-ATPase without causing denaturation.
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Report
(2results)
Research Products
(14results)
