• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular cloning and analysis of mutable genes in Japanese Morning Glory.

Research Project

Project/Area Number 60480002
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionThe University of Tokyo

Principal Investigator

KOMEDA Yoshibumi  Molecular Genetics Research Laboratory, 国立大学(その他), 講師 (10124215)

Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1986: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1985: ¥5,200,000 (Direct Cost: ¥5,200,000)
Keywordsmutability of Japanese Morning Glory / transposon / DNA断片の多型
Research Abstract

Many higher plants have phenotypic variegations. The genetic mutability is included in this class of phenotypes. Japanese scientists have studied extensively with Japanese Morning Glory and the four o'clock plant. In this study, the author is aiming to isolate and analyse possible mutable genes from Japanese Morning Glory. The first step was to purify higher molecular weight DNA from the plant. Two methods were emplyed for isolation of the genes; one is to examine genetic polymorphisms among strains of Morning Glory. The other is to detect DNA fragments carrying the structure of stem and loop. I found many polymorphisms among DNA samples of various horticultural strains by Southern hybridization. However, no correlation was detected between the mutability and the polymorphic pattern. one polymorphic pattern was difficult to be concluded by single insertional event.
Next, I tried to isolate transposon-like DNA fragments by the detection of stem and loop structures which are characteristic of transposons. The DNA of Morning Glory mutants was digested by DNase I and was denatured. The single stranded DNA was reannealed under the condition that makes intramolecular double strands. The fraction of single stranded DNA was digested by single strand specific nuclease. The resulted double stranded DNA was cloned into dG-tailed plasmid pUC9. A pool consisted of chimeric plasmids carrying cloned trasposon-like DNA. After the examination of the length and copy number of the inserts, representative five clones were sequenced. I detected an inverted repetitive sequence and the fragments that showed polymorphism among strains and that had about 60% homology to the stem of Tam1 a transposon of snap dragon).
Accordingly, the latter method was shown to be effective. I will further this study from two points; polymorphism and movability.

Report

(1 results)
  • 1986 Final Research Report Summary

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi