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Molecular mechanism of sperm activation by species specific egg jelly pepetides

Research Project

Project/Area Number 60480022
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionKanazawa University Faculty of Science (1986)
Teikyo University (1985)

Principal Investigator

SUZUKI Norio  Faculty of Science,Kanazawa University,Professor, 理学部, 教授 (20082133)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Masaaki  Faculty of Science,Kanazawa University,Research associate, 理学部, 助手 (60182458)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1986: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1985: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordssperm activating peptides / speract / mosact / alloresact / egg jelly / ウニ / 呼吸
Research Abstract

Species specific sperm activating peptides were purified and their amino acid sequences were determined. Mosact (Asp-Ser-Asp-Ser-Ala-Gln-Asn-Leu-Ile-Gly) and its two derivatives were obtained from the egg jelly of the sea urchin Clypeaster japonicus. Alloresact (Lys-Leu-Cys- Pro-Gly-Gly-Asn-Cys-Val) and its five derivatives were from Glyptocidaris crenularis egg jelly. Many new speract derivatives were purified from the egg jelly of sea urchins Hemicentrotus pulcherrimus, Anthocidaris crassispina, Pseudocentrotus depressus, Strongylocentrotus purpuratus and Lytechinus pictus. They are <Ser^(3,10)> -speract, <Ser^1> , <Ala^3> , <Gly^5> -speract, <Ser^(3,5,7)> , <Asp^(10)> -speract, <Ser^(3,5,7)> -speract, <Ala^3> , <Gly^5> -speract, <Ser^3> , <Thr^5> -speract, <Thr^5> , <Gln^(10)> -speract and Des- <Gly^1> -[ <Thr^5> , <Gln^(10)> ]-speract. Speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly) and its derivatives do not cross-react with mosact or alloresact and vice versa. Addition of mosact to C.japonicus spermatozoa resulted in the rapid appearance of a newly-stained protein on SDS-polyacrylamide gels as well as speract did to spermatozoa of sea urchins belonging to the order Echinoida and alloresact did to G. crenularis ( in the order Arbacioida ) spermatozoa. Sodium ions were essential for this.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Shimomura,H.;Suzuki,N.;Garbers,D.L.: Peptides. 7. 491-495 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Suzuki,N.;Yamaguchi,M.: Zoological Science. 3. 801-806 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yamamoto,R.;Ueta,N.;Suzuki,N.: Zoological Science. 3. 817-824 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Suzuki,N.;Kurita,M.;Yoshino,K.;Kajiura,H.;Nomura,K.;Yamaguchi,M.: Zoological Science. 4. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Suzuki,N.;Kurita,M.;Yoshino,K.;Yamaguchi,M.: Zoological Science. 4. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Suzuki,N.;Kajiura,H.,Kurita,M.;Yoshino,K.;Yamaguchi,M.: in preparation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Shimomura, H., Suzuki, N. and Garbers,D.L.: "Derivatives of speract are associated with the eggs of Lytechinus pictus sea urchins." Peptides. 7. 491-495 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Suzuki, N. and Yamaguchi, M.: "Species specific respiratory stimulation of sea urchin (Clypeaster japonicus) spermatozoa by an egg associated factor." Zoological Science. 3. 801-806 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yamamoto,R., Ueta, N. and Suzuki, N.: "Purification and characterization of cold-insoluble glycoproteins from porcine seminal plasma." Zoological Science. 3. 817-824 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Suzuki,N., Kurita,M., Yoshino,K., Kajiura,H., Nomura,K. and Yamaguchi,M.: "Purification and structure of mosact and its derivatives from the egg jelly of the sea urchins Clypeaster japonicus" Zoological Science. 4. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Suzuki,N., Kurita,M., Yoshino,K. and Yamaguchi, M.: "Speract binds exclusively to sperm tails and causes a mobility shift in a major sperm tail protein of sea urchins." Zoological Science. 4. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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