| Project/Area Number |
60480029
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | HIROSAKI University |
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Principal Investigator |
SAITO Ken-ichi Faculty of Agriculture,HIROSAKI Univ., Professor, 農学部, 教授 (70003423)
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| Co-Investigator(Kenkyū-buntansha) |
HIDANO Yutaka Faculty of Agriculture,HIROSAKI Univ.,Assistant Proffessor, 教育学部, 助教授 (90133850)
ASADA Takenori Faculty of Agriculture,HIROSAKI Univ.,Assistant Proffessor, 農学部, 助教授 (10003518)
NIIZEKI Minoru Faculty of Agriculture,HIROSAKI Univ.,Assistant Proffessor, 農学部, 助教授 (40001490)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1985: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | self-fruitfulness / parthenocarpy / self-compatibility / 単為結果性 / プロトプラストの融合 / パイオニア花粉 / 他動的単爲結果性 / 胚培養 |
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| Research Abstract |
1) In an examination of self-fruitfulness induced by artificial self-pollination, most of cultivars showed almost 0% of the self-fruitfulness except for cv. 'Raritan' which showed 50% of the selffruitfulness.
2) Sixty-four cultivars were tested for the parthenocarpy by spraying 400 ppm gibberelic acid. The results showed that thirty-one cultivars were capable of yielding mature parthenocarpic fruits. The fruits-set on an average was 18% and the highest fruit-set was 100% in cv. 'de011o'. The relationship between the self-fruitfulness and the parthenocarpy was also investigated. The correlation coefficient between them was significantly positive while a few cultivars showed the negative correlation.
3) From year to year, the repeatability value of self-fruitfulness caused by artificial selfpallination was relatively high(75%) but that of parthenocarpy was rather low (30%).
4) It was very hard to yield mature parthenocarpic fruits compared with seeded fruits, especially when the mean temperature in june was over 18C゜.
5) It was found that 'pioneer' pollens treatment following self-pollination significantly improved both fruit-and seed set in cv. 'Megumi'.
6) The protoplasts isolated from both nucellus calli of cv. 'Fuji' and shoot calli of cv. 'Starking Delicious' cultured in vitro. In addition, the protoplast fusion of two cultivars was carried out by somewhat modified method of Menczel and Wolfe (1984). The cell division of hybrid protoplasts occurred and they grew up to the calli.
7) It found that a minimum number of pollens required for the development of normal maturefruit was about fifty per a flower in cv. 'Ralls janet'. Significant difference between parthenocarpic - and seeded fruits could not find in qualities such as taste and storage ability in cv 'Megumi'
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