| Project/Area Number |
60480031
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | The Ishikawa Agricultural College |
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Principal Investigator |
NIIZEKI Hiroo Research Institute of Agricultural Resources, The Ishikawa Agricultural College, Professor, 農業資源研究所, 教授 (60140951)
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| Co-Investigator(Kenkyū-buntansha) |
KOBA Takato Research Institute of Agricultural Resources, The Ishikawa Agricultural College,, 農業資源研究所, 助手 (40170302)
SHIMADA Takiko Research Institure of Agricultural Resources, The Ishikawa Agricultural College,, 農業資源研究所, 助教授 (20170946)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1985: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | wild rice / Oryza alta / emmer wheat / Trit cum durum / young frolet culture / haploid / triploid / p-fluorophenylalanine / PFP |
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| Research Abstract |
Studies on the genome extraction of the DD genome plant from a wild rice, Oryza alta (2n=48,genome CCDD) and of theBB genome plant from emmer wheat, Triticum durum (2n=28, genome AABB) were carried out using the techniques of tissue culture and p-fluorophenylalanine(PFP) treatment.
In the case of a wild rice, triploid plants (2n=36, genome CCD or CDD) were obtained among plants regenerated from callus culture of young florets of O. alta (2n=48, tetrapolid plant) on the medium containing 50 mg/l of PFP. Trials to obtain the CC or DD genome plant from these triploid plants have not been succeeded so Far.
In the case of a emmer wheat, a haploid plant (2n=14, genome AB) were picked out among plants from seeds obtained by injection of PFP solution into glumes of T. durum (2n=28, tetraploid plant) at 6 hours after fertilization. Callus cells from this haploid plant after PFP treatment contain reduced number of chromosomes less than haploid number, but A or B genome plant have not been obtained so Far.
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