| Project/Area Number |
60480046
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物保護
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| Research Institution | Kyushu University |
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Principal Investigator |
WAKIMOTO Satoshi Fac.Agic., Kyushu University . Professor, 農学部, 教授 (30038286)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIUNTEN Hiroshi Fac. Agric., Miyazaki University. Associate Professor, 農学部, 助教授 (50124805)
IWAI Hisashi Fac. Agric., Kyushu University . Research Assistant, 農学部, 助手 (90183194)
TSUCHIYA Kenichi Fac. Agric., Kyushu university . Research Assistant, 農学部, 助手 (40150510)
MATSUYAMA Nobuaki Fac. Agric., Kyushu University . Associate Professor, 農学部, 助教授 (40108676)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1987: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1985: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Pseudomonas glumae / pathogenicity / physiological properties / serological properties / fluorescent antibody method / selective medium / ecology / プラスミド / イネ籾枯細菌病菌 / 検索 / 抗菌物質 / ニトロソグアニジン / 変異 |
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| Research Abstract |
The bacterial grain rot disease of rice caused by Pseudomonas glumae Kurita et Tabei was first found in Kyushu in 1955. The disease is wide spread not only in Southern Japan but also in Northern Japan causing serious yield loss by year. Recently the disease was known to occur in Korea and China. The pathogenic bacterium incites the rice seedling rot when infected grains were sawn in seed boxes followed by incubation under the humid conditions. In the paddy fields, the symptom of grain rot suddenly breaks out, without showing any symptoms on rice plants during rice growing stages.
To establish effective measures to control the disease, studies on the physiological and ecological properties of the pathogenic bacterium are urgently reguired. The results obtained during the period of research project (1985-1987) were summarized as follows:
(1) Collection of the pathogenic bacteria : Forty-seven strains of P. glumae were collected from various localities of Japan. They were freeze-dried for l
… More
ong term preservation.
(2) Pathogenicity of the strains : Some strains have lost their pathogenicity during successive transfer on the medium. Others showed strong pathogenicity but differentiation in pathogenicity was not confirmed.
(3) Serological specificity : Heat treated antigens of P. glumae were highly specific in serological properties. The anti-sera produced against heat treated antigens did not react with any abcteria other than P. glumae with some exceptions.
(4) Selective media : S-PG medium which is very effective for detecting P. glumae was devised.
(5) Productivity of antibacterial substances : It was found that P. glumae strains produced antibacterial substances effective to various plant pathogenic bacteria.
(6) Detection of plasmid : Wild strains of P. glumae have various plasmid of 8-135 Mdal. NTG- induced mutant strains were different in plasmid pattern from that of parent strain. No correlation was found between pathogenicity and plasmid pattern and function of these plasmid is still unknown.
(7) Ecology of the pathogenic bacterium : It was confirmed that P. glumae multiplied in pure water when inoculated at a concentration less than 10^6 cells/ml to survive for many years.
The bacterium was detected at the basal part of rice plant during growing seasons by S-PG medium. The mechnisms of bacterial invasion was also made clear by scanning and transmission type electron microscopes. Less
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