Studies on salt-tolerance mechanism and the role of membrane lipids in higher plants
| Project/Area Number |
60480055
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | Department of Agricultural Chemistry, Shimane University |
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Principal Investigator |
HIRAYAMA osamu Professor at Fuaculty of Agri., Shimane Univ., 農学部, 教授 (80032580)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Hideyuki Professor at Faculty of Agri., Shimane Univ., 農学部, 教授 (50032595)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1986: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1985: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Salt-tolerance / Halophytic Plant / Ion-transport / Prtificial Lipid Membrane / Membrane Lipids / ATPase |
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| Research Abstract |
The membrane lipids of six higher plants which differ in salt tolerance, were analyzed and compared. The root lipids increased in ratio of phospholipid/glycolipid with decreasing salt-tolerance. A similar increase in the ratio was observed with decreasing salinity of environment, when halophytic orach was exposed to varying salinity. Measurements of ion-transport rates with artificial lipid membranes revealed that the root lipids of salt-resistant plants formed more permeable membranes than those of salt-sensitive ones. This was concerned with a difference in the phospholipid/glycolipid ratios, because the former lipids were suppressive and the latter stimulative for the membrane permeability. The intact roots of orach and cucumber were measured for ion absorption rates using an improved method. The orach roots absorbed <Cl^-> at 5.4 times and <Na^+> at 6.4 times higher rates than the cucumber roots. The root membrane ATPases from halophytic orach and salt-sensitive cucumber were separated and characterized. The experiments suggest that the level of plasma-membrane ATPase in cucumber was much higher than that in orach roots. The present results make us explain that salt-resistant plants absorb excess external ions mostly in passive transport, while the roots of salt-sensitive plants selectively absorb external ions in active transport rather than in the passive one.
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Report
(1 results)
Research Products
(5 results)
