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Biochemical research on the recognition mechanism for trapping of nematode by nematophagous fungus.

Research Project

Project/Area Number 60480056
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 発酵・醸造
Research InstitutionUniversity of Tsukuba

Principal Investigator

YAMANAKA KEI  Institute of Applied Biochemistry, Professor, 応用生物化学系, 教授 (30035951)

Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsNematode-trapping fungus / Pine-wilt nematode / Recognition mechanism / Lectin / Hemagglutinin / Monoclonal antibody / Immunofluorescent staining / レクチンの抗体 / 粘着性多糖
Research Abstract

1 Two types of hemagglutinin(lectin) were purified from mycelium and cultural filtrate. They were identical by several aspects and double diffusion test with antibody of exocellular lectin. Molecular weight was estimated as 55,000 dalton and was consisted 4 identical subunits having molecular weight as 14,500. The lectin showed high affinity on polysaccharides from animal origin and nematodeextract (non-proteinous fraction).
2. Six clones of monoclonal antibodies were obtained from mouse splen cells.
3. Lectin was detected on the surface of hyphae with antibody and monoclonal antibodies by immunofluoescent technique.
4. Trapping of nematode by Arthrobotrys ellipsospora Y4007 was completely prevented by antibody. Lectin receptor in the nematode cuticle was demonstrated with monoclonal antibody by ELISA technique on disc gels.
5. Fusarium was found to produce potent hemagglutinin, and purified from F. graminearum No.15. Suar specificity of Fusarium lectin was closely similar with Arthrobotrys lectin. This lectin was also effective to prevent trapping.
6. Polysaccharide was betected on the trapping devise by pigment staining.
This polysaccharide also play important role for non-specific capture of nematode. SUMMARY: Lectin is evidently involved for the first recognition of nematode by this nematophagous fungus.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] K. Yamanaka;K. Wakabayashi and T. Saito: Agric. Biol. Chem.52(3). 675-683 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 宮沢靖史, 東眞彦, 山中啓: 第58回日本生化学大会(9.30, 1985)(同講演要旨集, 生化学). 57(8). 1139 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 長谷川成人, 真崎知生, 新井隆夫, 山中啓: 第58回日本生化学会大会(9.30, 1985)(同講演要旨集, 生化学). 57(8). 1139 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 青井達谷, 長谷川成人, 新井孝夫, 山中啓: 第9回糖質シンポジウム(7.21, 1986)日本炭水化物研究会(同講演要旨集). 9. 55-56 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K. Yamanaka;T. Okamoto and Y. Miyazawa: Agric. Biol. Chem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K. Yamanaka;Y. Miyazawa;N. Hasegawa;T. Higashi;T. Arai and T. Masaki: Agric. Biol. Chem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K,Yamanaka, K,Wakabayashi and T,Saito: "Capture of pine-wilt nematodes by Artrobotrys ellipsospora Y4007. Mucin-specific hemagglutinin and its role in the capture." Agric. Biol. Chem.52(3). 675-683 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T,Aoi, N,Hasegawa, T,Arai and K,Yamanaka,: "Properties and function of lectin from nematophagous fungus, Arthrobotrys ellipsospora Y4007." Abstracts of the 9th Symposium on Carbohydrate.9. 55-56 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K,Yamanaka,, T,Okamoto and Y,Miyazawa: "Purificationand properties of hemagglutinin of nematode-trapping fungus, Arthrobotrys ellipsospora Y4007." Agric. Biol. Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K,Yamanaka,, Y,Miyazawa, N,Hasegawa, T,Higashi, T,Arai and T,Masaki: "Lectin-mediated trapping of nematode by Arthrobotrys ellipsospora Y4007." Agric. Biol. Chem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 青井達也,長谷川成人,宮沢靖史,新井孝夫,山中啓: 第9回糖質シンポジウム講演要旨集. 9. 55-56 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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