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Regulation mechanisms of gene expression at the end of growth phase in Bacillus subtilis and the utilization to the production of useful substances

Research Project

Project/Area Number 60480057
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 発酵・醸造
Research Institutionthe University of Tokyo

Principal Investigator

SAITO Hiuga  Institute of Applied Microbiology, Univ.of Tokyo, Professor, 応用微生物研究所, 教授 (10013301)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Hirofumi  Institute of Applied Microbiology, Univ. of Tokyo, Assistant, 応用微生物研究所, 助手 (50175676)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥3,600,000 (Direct Cost: ¥3,600,000)
KeywordsBacillus subtilis / sporulation / gene expression / spo0A / spo0F / <BETA> -galactosidase, gene fusion / 発現調節
Research Abstract

Initiation of sporulation in Bacillus subtilis is controlled by spo0A, B, E, F, H, J and K genes. Among them, 0A has been seemed to be the most important gene because of its pleiotropic effects. Moreover, a suppressor mutation, sof-1, which recovered the sporulation of 0B, 0E and 0F mutants, was known to be an allele of spo0A; it implied that the altered 0A product alone could promote the sporulation in the absence of 0B, 0E and 0F products In the present studies, we determined the spo0A, 0B and 0F prorulation by weights as 29700, 24000, and 14229, respectively. Inhibition of sporulation by high copy 0F was also studied. It is of particular interest that the N terminal amino acid sequences of 0A and 0F products exhibited more than 50% homology. Since they are highly homologous also with Escherichia coli ompR and dye, it is probable that 0A and 0F products are transcription controlling factor responding to exogenous stimulations.
To examine the expression regulation of spo0A and 0F genes, we constructed 0A- or 0F-lacZ fusions; i.e. upstream sequences of 0A and 0F genes involving short N terminal sequences were fused in frame with the coding region of E.coli lacZ. The 0A-lacZ fusion expressed at a low level in the exponential growth phase culture. The expression was highly activated at the end of exponential growth, T0,and reached a plateau at T1. On the other hand, the expression of 0F-lacZ sharply rised up at T1 and the 0F product appeared to substitute the 0A product after T1. The T0 activation of 0A-lacZ expression required the functions of 0B, 0E, 0F and 0H products.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] J.A.Hoch;K.Trach;F.Kawamura;H.Saito: J.Bacteriol.161. 552-555 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.Oda;K.Yamada;R.Nomi;H.Shimotsu;H.Saito;Y.Kobayashi: Agric.Biol.Chem.49. 2025-2031 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] H.Yoshikawa;H.Sone;T.Seki;F.Kawamura;H.Saito: in Molecular Biology of Microbial Differentiation publ.American Society for Microbiology. SporeIX. 29-34 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] SaitoH.Yoshikawa;J.Kazami;S.Yamashita;T.Chibazakura;H.Sone;F.Kawamura;M.Oda;M.Isaka;Y.Kobayashi;H.: Nucleic Acids Research. 14. 1063-1072 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.Oda;M.Isaka;Y.Murooka;R.Nomi;H.Yoshikawa;F.Kawamura;H.Saito;Y.Kobayashi: Agric.Biol.Chem.50. 2845-2852 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] S.Yamashita;H.Yoshikawa;F.Kawamura;H.Takahashi;T.Yamamoto;Y.Kobayashi;H.Saito: Mol.Gen.Genet.205. 28-33 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] J.A.Hoch, K.Trach, F.Kawamura and H.Saito: "Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein" J. Bacteriol.161. 552-555 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.Oda,K.Yamada, R.Nomi,H.Shimotsu, H.Saito and Y.Kobayashi: "Identification of the product of prorulation gene spo0B in Bacillus subtilis" Agric. Biol. Chem.49. 2025-2031 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] H.Yoshikawa, H.Sone, T.Seki,F.Kawamura and H.Saito: "Functions of spo0F upstream region for the sporulation of Bacillus subtilis" in Molecular Biology of Microbial Differentiation pub.by: American Society for Microbiology. spore IX. 29-34 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] H.Yoshikawa, J.Kazami, S.Yamashita, T.Chibazakura, H.Sone, F.Kawamura, M.Oda, M.Isaka, Y.Kobayashi, and H. Saito: "Revised assignment for the Bacillus subtilis spo0F gene and its homology with spo0A and with two Escherichia coli gene" Nucleic Acids Research. 14. 1063-1072 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] N,Oda, M.Isaka, Y.Murooka, R.Nomi, H.Yoshikawa,F.Kawamura, H.Saito and Y.Kobayashi: "Purification of the early sporulation gene spo0F product of Bacillus subtilis" Agric. Biol. Chem.50. 2845-2852 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] S.Yamashita,H.Yoshikawa,F.Kawamura,H.Takahashi,T.Yamamoto, Y.Kobayashi and H.Saito: "The effect of spo0 mutations on the expression of spo0A- and spo0F-lacZ fusions." Mol. Gen. Genet.205. 28-33 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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