| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Research Abstract |
In view of the development of alternative sources of energy for liquid fuels, the ethanol production from renewable resouces has currently become important. Zymomonas mobilis is a facultative anaerobic gram-negative bacterium, and assumed to be useful for the commercial production of ethanol because of its higher productivity of ethanol compared with that of industrial yeast. However, there are some defects in Zymomonas, for instance the range of substrate utilized by the organism is restricted to glucose, fructose and sucrose. Therefore, extending this narrow range by genetic manipulation would increase this organism's usefulness and versatility for industrial uses.
In this imvestigation, with the aim of improving this defect by the recombinant DNA techniques, we have undertaken the genetic and biochemical studies of Zymomonas as follows; 1) Construction of shuttle vectors hetween Z. mobilis and E. coli, 2) Expression of foreign genes in Z. mobilis, 3) Construction of a promoter-cloning vector for Z. mobilis, and isolation of promoter DNAs from Z. mobilis chromosomal DNA, 4) Analysis of a promoter DNA of Z. mobilis, 5) Establishment of the transformation method of Z. mobilis, 6)Fermentation of lactose by Z. mobilis carrying a Lac^+ recombinant plasmid, 7) Metabolism of galactose of Z. mobilis, 8) Introduction and expression of E. coli-galactosidase gene in Z. mobilis, 9) Cloning of genes encoding the sucrose fermenting ability of Z. mobilis, 10) Purification and properties of sinble-stranded DNA-specific nuclease z_1 from Z. mobilis.
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