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Establishment of monoclonal antibodies ot the fish pathogens and it's appication for the rappid diagnosis of fish disease

Research Project

Project/Area Number 60480066
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionFaculty of Fisheries, Hokkaido Univeresity

Principal Investigator

KIMURA Takahisa  Professor, Faculty of Fisheries, Hokkaido University, 水産学部, 教授 (60001583)

Co-Investigator(Kenkyū-buntansha) YOSHIMIZU Mamoru  Instructor, Faculty of Fisheries, Hokkaido University, 水産学部, 助手 (40122915)
TAJIMA Kenichi  Instructor, Faculty of Fisheries, Hokkaido University, 水産学部, 助手 (80002252)
EZURA Yoshio  Associate Professor, Faculty of Fisheries, Hokkaido University (Yoshihara,Te), 水産学部, 助教授 (80001618)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordsモノクローナル抗体 / IPNV / IHNV / A.salmonicida / R.salmoninarum / 反応特異性 / 迅速診断法 / 魚類病原ウイルス / IPN / モノクローン抗体 / 認識抗原 / 迅速診断
Research Abstract

Monoclonal antibody technology makes if possible to analyse specific and crossreacting antigens among strains of a single bacteral or viral species, and could be used for immunodiagnosis.
One hybride secreting antibldy (C-1) to the VR-299 serotype of infectious pancreatic necrosis virus (IPNV) was selected from the fusion of myeloma cells and spleen cells from mice immunized with pelleted virus. The C-1 monoclonal antibody possessed the kappa (K) light chain isotype and had the gamma 1. (G1). C-1 monoclonal antibody showed the ability to neutralize the VR-299 serotype of IPNV. Using a fluorescent antibldy technique (FAT) and coagglutination test, C-1 monoclonal antibody was found to be broadly reactive against the representative three serotypes of IPNV, VR-299, Ab and Sp strains. By the radioimmunoprecipitation (RIP) and western blotting method, C-1 monoclonal antibody was reacted only with <beta> protein of IPNV induced polypeptide and structural polypeptide of virus virion.
C-1 monoclonal antibody could be used for the rappid diagnosis of IPN using the FAT and coagglutination test. C-1 monoclonal antibody sensitized staphylococci showed positive reaction with the filtrate antigen prepared from diseased fish within 30 min.
For other fish pathogens, 6 hybrids which produced the antibody against the A. salmonicida were established and the culture medium of these hybrids showed the reaction with only the homologous antigen by FAT.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 吉水守: 日本生産学会誌. 53. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 吉水守: 魚病研究. 23. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] YOSHIMIZU Mamoru: "C-1 monoclonal antibody against the %v%r-299 serotype of infectious pancreatic necrosis virus (IPNV)." Nippon Suisan Gakkaishi. 53. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] YOSGIMIZU Mamoru: "Rapid serological diagnosis of infectious pancreatic necrosis (IPN) using C-1 monoclonal antibody." Fish Pathology. 23. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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