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Metabolism of Highly unsaturated Fatty Acids to Prostaglandins and their Physiological Activities in Fish.

Research Project

Project/Area Number 60480069
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionHIROSHIMA UNIVERSITY, Faculty of Biological Science

Principal Investigator

KAYAMA Mitsu  Marine Biochemistry, Faculty of Biological Science, Hiroshima University, 生物生産学部, 教授 (90034446)

Co-Investigator(Kenkyū-buntansha) IIJIMA Noriaki  Marine Biochemistry, Faculty of Biological Science, Hitoshima University, 生物生産学部, 助手 (90136143)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1985: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsHuman / Carp / Rainbow Trout / Red Sea Bream / Blood / Platelets / Thrombocytes / AA / EPA / DHA / TX / PG / ドコサヘキサエン酸(DHA) / トロンボキサン(TX)
Research Abstract

It is well known that human platelets convert arachidonic acid (AA) to prostaglandins (PGs), especially to thromboxane A_2 (TXA_2), which is a potent inducer of blood aggregation. Although thrombocytes are considered to be the clotting cells of fish blood similar to mammalian platelets, the syntheses of PGs in fish thrombocytes still remain unclear. Therefore, the present study was designed to clarify the capacity of PG syntheses from exogenous AA, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) in carp, rainbow trout, and red sea bream thrombocytes, and compared with that in human platelets.
The blood of carp and rainbow trout was collected from dorsal aorta, and that of red sea bream from cuvierian duct. The thrombocyte-rich plasma (TRP) was prepared, mixed with N-hydroxyethylpiperazine-N'-2-ethanesufonic acid (HEPES) buffer, centrifuged, and resuspended. The washed pellets were incubated with ^<14>C-labelled highly unsaturated fatty acids (AA, EPA, and DHA).
Washed human platelets mainly converted AA to thromboxane B_2, however, washed carp and red sea bream thrombocytes converted AA to PGF_<2<alpha>>,PGE_2, and PGD_2, and washed rainbow trout AA to these three PGs and EPA to TXB_3. Accordingly, it is assumed that there are differences in PG syntheses between human platelets and fish thrombocytes and even among the fish species, Finally, it is considered that the differences in PG syntheses among human platelets and fish thrombocytes cause the different behaviors in the blood aggregation.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 鹿山光, 飯島憲章, 佐道哲也, 松本浩幸, 浅田敏雄, 五十嵐紀子, 柴忠明, 山口了三: 日本水産学会誌. 53(7). 1301 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 鹿山光, 佐道哲也, 飯島憲章: 日本水産学会誌. 52(5). 925 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 鹿山光, 佐道哲也, 飯島憲章, 浅田敏雄, 五十嵐紀子, 柴忠明, 山口了三, 平井利生: 日本水産学会誌. 51(11). 1911 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsu KAYAMA, Noriaki IIJIMA, Tetsuya SADO, Hiroyuki KATSUMOTO, Toshio ASADA, Michiko IGARASHI, Tadaaki SHIBA, and Ryozo @yAMAGUcHI: "Comparative Studies on Aggregation Bihaviors of Human, Carp, and Rainbow Trout Blood" Nippon Suisan Gakkaishi. 53(7). 1301- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsu KAYAMA, Tetsuya SADO, and Noriaki IIJIMA: "The Prostaglandin Syntesis in Rainbow Trout Thrombocyte" Nippon Suisan Gakkaishi. 52(5). 925- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsu KAYAMA, Tetsuya SADO, Noriaki IIJIMA, Toshio ASADA, Michiko IGARASHI, Tadaaki SHIBA, Ryozo YAMAGUCHI, and Toshio HIRAI: "The Prostaglandin Synthesis in Carp Thrombocyte" Nippon Suisan Gakkaishi. 51(11). 1911- (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsu Kayama: Bull Japan Soc Sci Fish. 51(11). 1911 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] Mitsu Kayama: Bull Japan Soc Sci Fish. 52(5). 925 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 鹿山光: "水産学シリーズ57"水産動物の筋肉脂質"(鹿山光編)13.魚類の高度不飽和酸とプロスタグランジン" 恒星社厚生閣, 149-161 (1985)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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