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Studies on fertilization in vitro and culture of mammalian eggs

Research Project

Project/Area Number 60480079
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionOkayama University (1986)
Kyoto University (1985)

Principal Investigator

NIWA Koji  College of Agriculture, Okayama University, 農学部, 助教授 (40089115)

Co-Investigator(Kenkyū-buntansha) YUHARA Masataka  College of Agriculture, Okayama University, 農学部, 助教授 (20032980)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1986: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1985: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsCattle / Goat / Rat / In-vitro fertilization / Eggs / Oocytes / Spermatozoa / 受精能獲得
Research Abstract

FERTILIZATION IN VITRO IN THE RAT. Newly ovulated eggs from immature rats induced to superovulate were inseminated in vitro with preincubated spermatozoa. A total of 84 two-celled eggs about 35 hr after insemination were transferred to the oviducts of 8 pseudopregnant rats on the first day of diestrus. Five recipients which had received a total of 59 eggs became pregnant and 9 male and 7 female newborn young were obtained (27% of development).
For the study of fertilization in vitro of rat oocytes matured in culture, oocytes with an intact germinal vesicle were cultured for 14-15 hr and exposed to preincubated spermatozoa. The proportion of the oocytes penetrated 21.5-46 hr after insemination was higher when they were cultured in a modified Krebs-Ringer bicarbonate (m-KRB) solution including fetal calf serum (FCS, 10 or 20%)(50-91%) than 4 mg bovine serum albumin (BSA)/ml (19-20%). The proportion of cleaved eggs 46 hr after insemination, however, was higher in the oocytes cultured in th … More e medium with BSA (90%) than FCS (50-52%).
FERTILIZATION IN VITRO OF BOVINE FOLLICULAR OOCYTES. Frozen semen obtained from 6 different bulls were washed twice with Brackett & Oliphant's medium (BO medium) including 10 mM-caffeine and preincubated for 5 hr at 39゜C after thawing. Bovine follicular oocytes cultured for 20-22 hr in a Hepes buffered TC-199 medium supplemented with FCS (10%) were introduced into the preincubated sperm suspension. The proportion of the oocytes penetrated 20-24 hr after insemination varied greatly according to the different bulls: very high penetration rates (83% in average) were obtained in one bull (P-157), but in other 5 bulls penetration rates were considerably low (0- 25%). These variations in penetration rates in vitro were found to be independent of conception rates to artificial insemination using the same semen samples. When the frozen semen from a P-157 bull was used, higher penetration rate of the oocytes was obtained after insemination with spermatozoa which were washed BO medium (80%) than m-KRB solution (45%) including caffeine. The posibilities of fertilization in vitro of goat eggs and development in culture of cattle oocytes fertilized in vitro can be resolved by further experimentation. Less

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] NIWA,K.: Theriogenology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Niwa, K.: "Variations in penetration rates in vitro of bovine follicular oocytes do not reflect conception rates to artificial insemination using frozen semen from different bulls" Theriogenology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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