| Project/Area Number |
60480087
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎獣医学
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| Research Institution | Faculty of Agriculture |
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Principal Investigator |
KANOE Masamitsu Faculty of Agriculture, Yamaguchi University, Professor, 農学部, 教授 (00035099)
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| Co-Investigator(Kenkyū-buntansha) |
KAI Kazushige Faculty of Agriculture, Yamaguchi University,Assoc,Prof., 農学部, 助教授 (60085628)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Necrose bacillus / Leukocidin / Cytotoxic substances / Fusobacterium necrophorum / フソバクテリウム / 溶血毒 / 感染防御抗原 / 壊死桿菌 / 第一胃 / 肝臓 |
|---|
| Research Abstract |
Partial purification and cytotoxic effects of Fusobacterium necrophorum leukocidin on bovine different cells were investigated.
F. necrophorum leukocidin in diffusate of dialysis culture was partially purified by ammonium sulfate saturation and by ultrafiltration. It was free from the bacterial hemolysin and additional toxic substances.
Scanning electron microscopy revealed diminution and loss of the microvilli and destruction of the leukocidin-treated ruminal and hepatic cells. Bovine peripheral granulocytes were completely destroyed by the toxin. In lymphocytes the leukocidin induced loss of microvilli and irregular shapes of the affected cells.
Bovine anti leukocidin was measured by the ELISA test using the leukocidin. These findings indicate that the leukocidin appeared to be associated with the virulence factors, playing role in the pathogenesis of necrobacillosis.
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