| Project/Area Number |
60480096
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
KAWAKAMI Yoshimi Rakuno Gakuen Univ. Professor, 酪農学部, 教授 (90048075)
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| Co-Investigator(Kenkyū-buntansha) |
KIRISAWA Rikio Rakuno Gakuen Univ. Assistant Prof., 酪農学部, 講師 (70153252)
ONUMA Misao Rakuno Gakuen Univ. Associate Prof., 酪農学部, 助教授 (70109510)
SONODA Mitsuo Rakuno Gakuen Univ. Professor, 酪農学部, 教授 (50001516)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | Monoclonal antibodies / Bovine leukosis / Tumor associated antigen / モノクローナル抗体 / 血清診断 |
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| Research Abstract |
Monoclonal antibodies against tumor-associated antigens(TAAs) expressed in the leukemia cells of enzootic bovine leukosis(EBL) were examined for their possible usefulness of in detecting EBL tumor cells. The antibodies immunohistochemically reacted with EBL tumor cells and had cytotoxic activity against the cells but not against normal adult bovine peripheral blood lymphocytes(PBL). The TAA was also found in several samples of PBL taken from BLV-infected healthy cattle (8 positive out of 81 tested), as well as in PBL from 13 out of 14 cattle with lymphocytosis but no evidence of tumor.
Seven cows with TAA positive in their PBL but no clinical signs of EBL were killed for histopathological observations. Of 7 cows, 4 had tumor masses, 2 showed proliferation of neoplastic lymphoid cells histologically and one had splenomegaly.
These resutls indicated that monoclonal antibodies against TAAs might be a useful tool not only for diagnosis EBL but also for screening of BLV-infected cattle with the potential to develop tumors in future.
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