Distribution of labeled amino acids in mice studied by whole- body autoradiography and biochemical analysis.
| Project/Area Number |
60480102
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Osaka Medical College |
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Principal Investigator |
KIHARA Takashi Department of Anatomy, Osaka Medical College, 医学部, 教授 (60084802)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Masahito Department of Anatomy, Osaka Medical College, 医学部, 助教授 (70084902)
GOTO Hideyuki Department of Anatomy, Osaka Medical College, 医学部, 助手 (20142656)
松島 正明 大阪医科大学, 医学部, 助手 (20131352)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1986: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1985: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | Labeled amino acid / amino acid metabolism / whole-body autoradigraphy / 高速液体クロマトグラフィー / ロイシン / ヒスチジン / アルギニン |
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| Research Abstract |
The distribution of lageled amino acids and their metabolites in mice were investigated by whole-body autoradiography and biochemical analysis. The ^<14>C- labeled amino acids used were tryptophan, tyrosine, leucine, histidine, arginine and GABA. They were intravenously injected into the mice. Cryosectioning of mice and whole-body autoradiography were carried out the modified Ullberg methods. For densitometry of autoradiographs, a computer system was introduced and radioactivities in organs and tissues were measured in detail. Distribution of tryptopnan, tyrosine, leucine, histidine and arginine had a common pattern. The highest radioactivity was observed in the pancreas, and relatively high radioactivities were in the liver, kidney, stomach, intestine and salivary glands and it was low in the brain and testis. However, the distribution of GABA had a different pattern presenting a considerable low radioactivity in the pancreas compared with the liver and kidney.
On the other hand, fractionation of the removed organs showed that the activity in the acid-soluble fractions decreased with time, but the activity in the acid-insoluble fractions increased. HPLC of the acid-soluble fractions revealed that radioactive metabolites and/or their molar ratios were different by the organs, this suggesting the organ specificity of the amino acid metabolism.
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Report
(2 results)
Research Products
(8 results)
