| Project/Area Number |
60480118
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Tokyo Metropolitan Institute for Neurosciences |
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Principal Investigator |
KAWAI Nobufumi Tokyo Metropolitan Institute for Neurosciences, 病態生理, 副参事 (00073065)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMAZAKI Kuniko Tokyo Metropolitan Institute for Neuroscinces, 病態生理, 研究員 (40142153)
MIWA Akikio Tokyo Metropolitan Institute for Neurociences, 病態生理, 研究員 (60142155)
SAITO Mitsuyoshi Tokyo Metropolitan Institute for Neurosciences
YOSHIOKA Masanori Setsunan University (30012652)
PAN-HOU Hidemitsu Setsunan University
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1987: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | glutamate / glutamate receptor / synaptic transmission / spider toxin Joro spider toxin / JSTX / GlutamateB / グルタミン酸レセプター / クモ毒 / JSTX海馬 / スライス標本 / NMA |
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| Research Abstract |
We have found that a spider ( Nephila Clavata, Joro spider) venom contains a potent inhibitor of the glutamate receptors. The toxin purified from the venom of Joro spider (JSTX) blocks both the excitatory postsynaptic potentials and glutamate-induced excitation in vertebrate as well as invertebrate synapses. Chemical characterization of JSTX revealed a unique structure containing 2,4-dihydroxyphenylacetylaspargine combined with various types of polyamines. Using synthetic compounds of the spider toxin and various type of analogs the structure-activity relationship of these compounds and blocking action was studied on the glutamate receptors. We have fond that 2,4 dihydroxyphenylacetyl-asparaginayl-cadaverine was essential for revealing the suppressive action on the glutamate receptor, whereas the remaining part containing various polyamine chains was responsible for binding of the toxin molecule to receptor-ionchannel complex.
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