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Functional role of lipoxygenase metabolism in cerebral microvessels

Research Project

Project/Area Number 60480128
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionFaculty of Pharmaceutical Sciences, Osaka University

Principal Investigator

IWATA Heitaroh  Fac. Pharm. Sci. Osaka Univ., Prof., 薬学部, 教授 (30028823)

Co-Investigator(Kenkyū-buntansha) MATSUDA Toshio  Fac. Pharm. Sci. Osaka Univ., Instructor, 薬学部, 助手 (00107103)
BABA Akemichi  Fac. Pharm. Sci. Osaka Univ., Assit. Prof., 薬学部, 助教授 (70107100)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥5,900,000 (Direct Cost: ¥5,900,000)
KeywordsCerebral microvessels / Lipoxygenase / Arachidonic acid metabolism / HETE / プロテインキナーゼ
Research Abstract

1. 12-Lipoxygenase (12-LPG) of cerebral microvessels (MV):
We found soluble and membrane-bound 12-LPG activities in rat cerebral MV. Their characteristics are as follows: Among the various neurotransmitter candidates, epinephrine and 5-HT inhibited the 12-LPG activity in which the inhibition was not related to their receptor mechanisms. 12-LPG activity ofMV was increased by 60% in streptozotocin-induced diabetic rats. 12-LPG of platelet was not affected in diabetic rats. 12-LPG of MV was not affected by adrenalectomy.
12-HETE, a product of 12-LPG reaction, had no effect on adenylate cyclase, angiotensin converting enzyme and gamma GTP activities of MV. We found that MV had cyclic AMP-dependent protein kinase (A-kinase) and calcium, phospholipid-dependent protein kinase (C-kinase) activities. 12-HETE inhibited the activity of A-kinase but not of C-kinase. In addition, retinoids markedly inhibited the A-kinase activity of MV.
2. Inhibitory facator of MV for platelet 12-LPG:
We found a inhibitory factors in MV for platelet 12-LPG activity. We characterized this inhibitory factor; the factor is heat-resistant high molecule. It may be protein which has heme molecule in its structure.
3. 12-LPG in rat platelet:
In comparison with 12-LPG of MV, we found several characteristics of platelet 12-LPG, especially the interaction of the enzyme with calcium.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] A.BABA,M.;KIMOTO,T.TATSUNO;T.INOUE;H.IWATA: Biochem.Biophys.Res.Comm.127. 283-288 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 馬場明道,岩田平太郎: 生産と技術. 37. 51-53 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] A.BABA;T.INOUE;S.SAKUMA;H.IWATA: in preparation. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] A.BABA;T.INOUE;S.SAKUMA;H.IWATA: (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] A.BABA;T.INOUE;H.IWATA: (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] A.BABA;S.SAKUMA;H.IWATA: (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] A. BABA, M. KIMOTO, T. TATSUNO AND H. IWATA: "Membrane-bound lipoxygenase of rat cerebral microvessels" Biochem. Biophys. Res. Comm.127. 283-288 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] A. BABA AND H. IWATA: "Lipoxygenase of cerebral microvessels (in Japanese)" Seisan to Gijutu. 37. 51-53 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] A. BABA, T. INOUE, S. SAKUMA AND H. IWATA: "12-Lipoxygenase activity of cerebral microvessels of diabetic rats" in preparation. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] A. BABA, T. INOUE, S. SAKUMA AND H. IWATA: "Inhibitory factors in cerebral microvessels for rat platelet 12-lipoxygenase" in preparation. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] A. BABA, T. INOUE AND H. IWATA: "Glutamate binding site in rat cerebral microvessels" in preparation. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] A. BABA, S. SAKUMA AND H. IWATA: "Binding of soluble 12-lipoxygenase to platelet membranes" in preparation. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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