| Project/Area Number |
60480128
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Faculty of Pharmaceutical Sciences, Osaka University |
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Principal Investigator |
IWATA Heitaroh Fac. Pharm. Sci. Osaka Univ., Prof., 薬学部, 教授 (30028823)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Toshio Fac. Pharm. Sci. Osaka Univ., Instructor, 薬学部, 助手 (00107103)
BABA Akemichi Fac. Pharm. Sci. Osaka Univ., Assit. Prof., 薬学部, 助教授 (70107100)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥5,900,000 (Direct Cost: ¥5,900,000)
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| Keywords | Cerebral microvessels / Lipoxygenase / Arachidonic acid metabolism / HETE / プロテインキナーゼ |
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| Research Abstract |
1. 12-Lipoxygenase (12-LPG) of cerebral microvessels (MV):
We found soluble and membrane-bound 12-LPG activities in rat cerebral MV. Their characteristics are as follows: Among the various neurotransmitter candidates, epinephrine and 5-HT inhibited the 12-LPG activity in which the inhibition was not related to their receptor mechanisms. 12-LPG activity ofMV was increased by 60% in streptozotocin-induced diabetic rats. 12-LPG of platelet was not affected in diabetic rats. 12-LPG of MV was not affected by adrenalectomy.
12-HETE, a product of 12-LPG reaction, had no effect on adenylate cyclase, angiotensin converting enzyme and gamma GTP activities of MV. We found that MV had cyclic AMP-dependent protein kinase (A-kinase) and calcium, phospholipid-dependent protein kinase (C-kinase) activities. 12-HETE inhibited the activity of A-kinase but not of C-kinase. In addition, retinoids markedly inhibited the A-kinase activity of MV.
2. Inhibitory facator of MV for platelet 12-LPG:
We found a inhibitory factors in MV for platelet 12-LPG activity. We characterized this inhibitory factor; the factor is heat-resistant high molecule. It may be protein which has heme molecule in its structure.
3. 12-LPG in rat platelet:
In comparison with 12-LPG of MV, we found several characteristics of platelet 12-LPG, especially the interaction of the enzyme with calcium.
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