| Project/Area Number |
60480155
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | School of Medicine, Tokai University |
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Principal Investigator |
TAMAOKI Norikazu School of Medicine, Tokai University, 医学部, 教授 (50055860)
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| Co-Investigator(Kenkyū-buntansha) |
HABU Sonoko School of Medicine, Tokai University, 医学部, 助教授 (30051618)
HATA Jun-ichi School of Medicine, Tokai University, 医学部, 教授 (90051614)
TADA Nobuhiko School of Medicine, Tokai University, 医学部, 講師 (80167494)
UEYAMA Yoshito School of Medicine, Tokai University, 医学部, 講師 (30072408)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1986: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1985: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | Hemopoietic factor / Colony stimulating factor / G-CSF / B cell growth factor <I> / Human tumor / ヒトがん / ヌードマウス |
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| Research Abstract |
For studying the pathogenesis of leukocytosis occasionally associated with neoplasia, 11 lines of human tumor transplantable to nude mice were established from cases with neoplasia and leukocytosis more than <10^4> / <mm^3> . These tumors included carcinoma originated from oral cavity, thyroid, lung and pancreas, leiomyosarcoma and myeloblastoma associated with CML and the extracts of these tumors showed CSF activity in vitro. A granulocyte colony stimulating factor (G-CSF) purified from the culture cell line of above oral cavity carcinoma showed prominent neutrophilia in vivo as well as G-CSF activity in vitro. The rabbit antibody to G-CSF which neutralized CSF activity in vitro demonstrated reaction products within endoplasmic reticulum and perinuclear space of original cell line. These findings indicates that G-CSF produced by human tumors directly stimulates host bone marrow cells and induces neutrophilic granulocytosis. In addition, nude mice bearing myeloblastoma and leiomyosarcoma showed prominent increase in splenic lymphocytes, B cells, plasma cells and serum IgM level. The supernatant of myeloblastoma in short term culture showed BCGF- <I> activity to mouse B cells triggered by anti- <mu> antibody.
Above results demonstrate that human neoplasms are able to produce various growth and differentiating factors to myeloid and lymphoid series and the human tumors transplantable to nude mice provide useful research resources for the study of hemopoietic factors produced by neoplasms.
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