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Diversity of functions and surface structures of Trypanosoma cruzi trypomastigotes

Research Project

Project/Area Number 60480161
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionInstitute of Tropical Medicine, Nagasaki University

Principal Investigator

KANBARA Hiroji  Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, 熱帯医学研究所, 教授 (20029789)

Co-Investigator(Kenkyū-buntansha) YANAGI Tetsuo  Department of Protozoology, Institute of Tropival Medicine, Nagasaki University, 熱帯医学研究所, 助手 (10174541)
NAKAZAWA Shusuke  Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, 熱帯医学研究所, 助手 (20180268)
FUKUMA Toshinide  Department of Protozoology, Institute of Tropical Mediccine, Nagasaki University, 熱帯医学研究所, 助教授 (90125146)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1985: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsTrypanosoma cruzi / trypomastigote / virulence / surface antigen / 表面抗原 / 表面構成成分 / トリポマスティゴート
Research Abstract

1. Virulence of Trypanosoma cruzi trypomastigotes is not caused by their penetrating abiliti but is dependent on their speed of the following steps. High-virulent trypomastigotes penetrated mouse macrophages or newly cultured fibroblasts from newborn mice at the almost same speed as low-virulent ones, but they promptly change form and start proliferation, while low-virulent ones require more than 48 hours lag time before proliferation. Tthis phenomenon is not found in the infectious experiment to the established fibroblasts (L-cells).
2. There are more than 30 protein antigens on the cell surface of trypomastigote, none of which are prominent in quality and quantity. Thys, immune mouse sera can not agglutinate living trypomastigotes, even though they show considerable titer of indirect immunofluorescence to them.
3. Lipid-like compoments predominantly appear on high-virulent trypomastigotes. Separated compoments from living trypomastigotes of different virulence by trypsin treatment are comparatively analysed on SDS-PAGE. White precipitates with a Mr range 37,000 to 50,000 were shown when the gels were fixed in 12.5% TCA but they disappeared when the samples were pre-treated with acetone.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 神原廣二: Biken Journal. 28. 71-77 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] エディタ・マ・ヘラモスラ: Tropical Medicine. 27(4). 211-219 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 神原廣二: Tropical Medicine. 28-2. 123-127 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 神原廣二: zbl. Bakt. Hyg. A. 264. 407-413 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 神原廣二: Tropical Medicine. 29(2). 123-126 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 神原廣二: Tropical Medicine. 29(3). 181-185 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanbara, H. & Nakabayashi, T.: "Comparative studies on surface antigenicity of Trypanosoma cruzi trypomastigotes from infected mouse blood and infected L-cell cultures" Biken Journal. 28. 71-77 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hermosura, Ma. E.: "Comparative study on high and low virulent tryoimastigotes of Trypanosoma cruzi: Infectivity to mouse macrophages, L-cells and newly cultured fibtoblasts from mouse heart" Tropical Medicine. 27 (4). 211-219 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanbara, H., Kondo, G. & Yanagi, T.: "Fibronectin attachment to different stages of Trypanosoma cruzi" Tropical Medicine. 28 (2). 123-127 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanbara, H., Yanagi, T., Nakazawa, S. & Fukuma, T.: "Comparison of virulent trypomastigotes with low-virulent ones derived from a single strain of Trypanosoma cruiz: infectivity to new fibroblasts from various organs of the mouse" Zbl. Bakt. Hyg. A. 264. 407-413 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kanbara, H., Fukuma T., Yanagi, T. & Nakazawa, S.: "Homogeneity of Trypamosoma cruzi strains of different virulence derived from a single strain" Tropical Medicine. 29 (2). 123-126 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 神原廣二: 熱帯医学. 28. 123-127 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 神原廣二: Zentralblatt fur Bacterrlogie Mikrobiologie und Hygiene. A264. 407-413 (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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