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A hypothesis as to mechanism of perturbation in biomembrane due to endotoxin. Participation of lysosomal cationic proteins with an endotoxin binding capacity

Research Project

Project/Area Number 60480167
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionIeate Medical University, School of Medicine

Principal Investigator

YOSHIDA Masao  Iwate Medical University, School of Medicine, Professor, 医学部, 教授 (50048229)

Co-Investigator(Kenkyū-buntansha) TSUNODA Nobuko  Iwate Medical University, School of Medicine, Assistant, 医学部, 助手 (90128926)
INADA Katsuya  Iwate Medical University, School of Medicine, Lecturer., 医学部, 講師 (80048446)
HIRATA Michimasa  Iwate Medical University, School of Medicine,Lecturer., 医学部, 講師 (20048359)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsEndotoxin / Granulocytes / Cationic protein / Mode of action / Biomembrane / Endotoxin binding protein / Hemagglutination / LPS / 生体膜障害 / リソゾーム / 赤血球凝集反応 / 抗菌活性
Research Abstract

1) Fractionation of cationic proteins (CAP's); CAP's were fractionated from rebbit peritoneal granulocytes through the steps of 0.1 M citric acid extraction, ethanol precipitation and gel filtration by FPLC. Since CAP's were capable of binding with heparin, a purification using heparin Sepharose is being investigated.
2) CAP's agglutinated the erythrocytes sensitized with LPS, especially Re-LPS (CAP-HA); CAP-HA was inhibited by preincubation of CAP with LPS.
3) CAP bound with LPS, especially Re-LPS in water solution, and made increased turbidity; the increase of turbidity depended upon CAP-levels, although the binding took place at an optimal ratio of both CAP and LPS levels; Low ionic strength condition and pH 6-7 were optimal to CAP-HA; the binding was reversible. This seems to be involved in both ionic and hydrophobic bounds of CAP and LPS. CAP-HA is thought to result from the binding mentioned above.
4) CAP exhibited antibacterial activity against S and R form gram-negative bacteria as well as gram-positive bacteria, expecially against R from gram-negatives. The activity seems to be bacteriolytic.
5) CAP bound with heparin made increased trubidity, consequently the binding neutralized mutually the properties of CAP and heparin.
6) One CAP fraction of less than 10,000 M.W. had high anticoagulant activity compared to other properties.
7) It seems that CAP can promote the activity of LPS, which bound with cells such as erythrocytes, and membrane perturbation.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 平田陸正, 角田伸子, 吉田昌男, 芝哲夫, 楠本正一: 血液と脈管. 17. 72-74 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 平田陸正, 角田伸子, 稲田捷也, 吉田昌男: 血液と脈管. 16. 480-489 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 吉田昌男: 化学と薬学の教室. 95. 74-78 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.Yoshida;M. Hirata;K. Inada;Z. Suzuki;J. Sasaki.: J. Iwata med. Ass.38. 375-381 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 吉田昌男: 免疫薬理. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 平田陸正, 角田伸子, 吉田昌男: 血液と脈管. 18. 592-594 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirata,Michimasa: "Increased thromboplastin activity of bone marrow cells due to lipid A analogs synthetized." Blood & Vessel. 17. 72-74 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirata,Michimasa: "Endotoxic DIC and tissue thromboplastin activity of bone marrow cells." Blood & Vessel. 16. 480-489 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yoshida,Masao: "A story of pyrogen." School of Chemistry and Pharmacy. 95. 74-78 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yoshida,Masao: "Necrotic alterations of bone marrow cell elements in severe gram negative bacterial infection." J. Iwate med. Ass.38. 375-381 (1976)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yoshida,Masao: "Is there the receptor against endotoxin ?" Immunopharmacology. in press. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.Hirata;N.Tsunoda;K.Inada;M.Yoshida: J.Immunol.Immunopharmacology. 【VI】. 171 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 平田陸正,角田伸子,稲田捷也,吉田昌男: 第33回毒素シンポジウム予講集. 33. 134-139 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 平田陸正,角田伸子,稲田捷也,吉田昌男: 日本細菌学雑誌. 41. 354 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 平田陸正,角田伸子,吉田昌男: 血液と脈管. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 角田伸子,平田陸正,吉田昌男: 日本細菌学雑誌. 42. 425 (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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