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Study on adsorption and penetration of vaccinia virus.

Research Project

Project/Area Number 60480171
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionNiigata University,

Principal Investigator

ICHIHASHI Yasuo  Associate Professor, Niigata University School of Medicine, 医学部, 助教授 (80027317)

Co-Investigator(Kenkyū-buntansha) OIE Masayasu  Assistant, Niigata University School of Medicine, 医学部, 助手 (70108017)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1987: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1986: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1985: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsVaccinia virus / Infection mechanism of verus / 細胞膜プロテアーゼ / 細胞内侵入機構 / モノクローナル抗体 / ウイルスレセプター
Research Abstract

Adsorption and penetration steps of virus infection determine viral tropism and disease patterns. The adsorption is achieved by interaction between viral ligand and host cell receptor, and penetration occurrs through fusion between viral and cellular membranes following adsorption or after phagocytosed. However, little is known about the molecular events of these processes.
We analyzed the phenomena related to the vaccinia virus infection that the infectivity of vaccinia virus can be increased by in vitro treatment of the virus with proteolytic enzyme or with phosphatidylserine. These activation of the virus infectivity seem to occur also in vatural infection process on host cell surface. We made several neutralizing monoclonal antibodies (MAb) which are specifically reactive against viral surface proteins. All of the MAbs did not significantly affect on absorptive efficiency of the virus to the cell surface, but showed differential neutralizing titers according to the activated state of the virus. The reactivity of the MAbs against the virus before and after the ativating treatments revealed that the proteolytic treatment cleaves VP54K to TVP41K, and PS-treatment changes epitope structure of VP3jK. An anti-idiotypic antibody against anti-VP3iK and/or VP32K was found to mediate activation of the virus through interaction with 32K Vero cell receptor. We concluded that vaccinia virus penetration took place through multi-step reaction. Viral surface proteins exist in a disulfide-linked complex, and each protein performs specific reaction of the process.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 大家正泰: Virology. 157. 499-459 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 市橋康夫: Virology in print. 163. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 市橋康夫: Virology. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 市橋康夫: 病態生理in print. 7. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 大家正泰: Virology. 142. 299-306 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masayasu Oie: "Modification of vaccinia virus penetration proteins analyzed by monoclonal antibodies." Virology. 157. 449-459 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yasuo Ichihashi: "Epitope mosaic on the surface proteins of orthopoxviruses." Virology. 163. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yasuo Ichihashi: "Virus-receptor interaction revealed by enhancement of infectivity with anti-idiotype-like antibody." Virology. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masayasu Oie: "Peversible inactivation and reactivation of vaccinia virus by manipulation of viral lipid composition." Virology. 142. 299-306 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 大家正泰: Virology. 142. 299-306 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] 大家正泰,市橋康夫: Virology. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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