| Project/Area Number |
60480174
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Kyoto University |
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Principal Investigator |
YODOI Junji Faculty of Medicine, Kyoto University, 医学部, 助手 (80108993)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1986: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Fc receptors / Fc <epsilon> receptor / IgE binding factor / Regulation of IgE production / Animal lectins / IL-2 receptor / IL-2 receptor inducers / IL-2レセプター誘導因子 |
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| Research Abstract |
To proceed the study on the structure and biological function of lymphocyte Fc <epsilon> receptor (Fc <epsilon> R) and IgE binding factor (IgE BF), we established a hybridoma-producing monoclonal anti-human Fc <epsilon> R (H107 moAb). By using H107 moAb, we purified 43KD Fc <epsilon> R from RPMI8866 cell lysate and 25KD soluble Fc <epsilon> R in the culture supernatant. Since the soluble Fc <epsilon> R showed IgE binding activity and in vitro IgE potentiating activity, it had characteristics of IgE BF. It was demonstrated that Fc <epsilon> R was degraded proteolytically to soluble IgE BF. We elucidated the N terminal partial amino acid sequence of 25KD soluble Fc <epsilon> R (IgE BF) and synthetized the oligo DNA probe. Using this probe, we cloned the Fc <epsilon> R. cDNA from the cDNA library of RPMI8866 cells. When Cos-7 cells were transfected with Fc <epsilon> R.cDNA, Fc <epsilon> R was expressed on the cell surface and IgE BF was released in the culture supernatant. Fc <epsilon> R was highly homological to animal lectins, including asialoglycoprotein receptor.
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