| Project/Area Number |
60480175
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Kyoto University |
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Principal Investigator |
KATSURA Yoshimoto Chest Disease Research Institute, Kyoto University. Professor, 結核胸部疾患研究所, 教授 (90027095)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIKAWA Shin-ichi Chest Disease Research Institute, Kyoto University. Assistant Professor, 結核胸部疾患研究所, 助教授 (60127115)
KINA Tatsuo Chest Disease Research Institute, Kyoto University. Assistant, 結核胸部疾患研究所, 助手 (30127071)
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| Project Period (FY) |
1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1986: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1985: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | Hemopoietic stem cell / Differentiation / T細胞 |
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| Research Abstract |
Differentiation of T cells from the hemopoietic stem cell was investigated. Since the stem cells have never been quantitatively analysed, we first deviced a method to quantity the stem cells for T cell lineage. C57BL (B6) mice were used as the recipient, and B10Thy1.1 mice were used as the donor. The recipients were either part-body-shielded (leg and tail, or thymus area) and irradiated (900 R) or whole body irradiated (800 R) and reconstituted with <10^7> syngeneic bone marrow cells. Donor cells were transferred eithter intravenously (i.v.) or intrathymically (i.t.). I.t. transfer method was much more (10 to 100 times) efficient for T cell generation than i.v. transfer method. By i.t. transferring serial dilutions of cells from various organs of B10Thy1.1 mouse, the frequency of the stem cells which have a potential to give rise to T cells (T-stem cells) was determined. The results are as follows: bone marrow, 1/8 x <10^3> ; fetal liver, 1/1.6 x <10^4> ; spleen 1/4.5 x <10^5> ; thymus, 1/7 x <10^5> .
In the recipients given a limited number of bone marrow cells, it was shown that the donor type T cells were the progeny of a single stem cells. Both helper T cells and killer T cells were found to be derived from a single stem cell. We further showed that the repertoire diversification of T cells occurred at random.
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