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Preparation of human monoclonal antibodies using human B cell producing autoantibodies and analyses of novel autoantigens.

Research Project

Project/Area Number 60480204
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionSapporo Medical College

Principal Investigator

YACHI Akira  Sapporo Medical College, Department of Medicine, 医学部, 教授 (50045324)

Co-Investigator(Kenkyū-buntansha) HINODA Yuji  Sapporo Medical College, Department of Medicine, 医学部, 助手 (10165128)
IMAI Kohzoh  Sapporo Medical College, Department of Medicine, 医学部, 講師 (60117603)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1985: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsAutoantibody / Autoantigen / Human monoclonal antibody / エピトープ
Research Abstract

In some cases with autoimmune diseases and malignancies, autoantibodies to unknown antigens were detected in the sera. Using monoclonal antibodies, we have studied these autoantigens and autoantibodies and analysed the immune system. In order to prepare human type monoclonal antibodies, human B cells from cases with autoimmune diseases and malignancies were used. Therein we experienced a colonic cancer patient with anti-CEA monoclonal antibody. For parent cells, human myeloma cell line ARH77, human plasma cell line UC729-6 and mouse myeloma cell line P3-X63-Ag8-653 were served. Human cell lines which we used for fusion gave little hybridomas, but many human monoclonal antibodies were obtained when P3-X63-Ag8-653 was used. By a fusion with B cells in pleural effusion of a gastric cancer patient, human monoclonal antibody 3B7 (IgG) was raised, showing a staining reactivity with gastric and colonic cancers but not with non cancerous tissues. Molecular profile of autoantigen to which monoclonal antibody 3B7 reacts is under a further investigation. At present we are trying to raise hybridomas from new parent cells KR-4 and KR-12 using a newly developed electrofusion method.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Yachi,A.,et al.: Jpn.J.Med.25. 127-134 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yachi,A.,et al.: Protides of Biological Fluids. 34. 355-358 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kitagawa,A.,Yachi,A.,et al: Jpn.J.Cancer Res.(Gann). 77. 922-930 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Sugiyama,T.,Yachi,A.,et al: Jpn.J.Cancer Res(Gann). 77. 722-729 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yachi,A.,Imai,.K.,et al Kano,K.ed.: "Labeled Antibodies"Tokyo Uhiversity Press. 429-432 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Imai,K.,Yachi,A.,et al Kano,K.ed.: "Labeled Antibodies"Tokyo University Press. 433-436 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Imai,K.,et al.: Gastroenterology. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hinoda,Y.,et al: Jpn.J.Cancer Res.(Gann). (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yachi,A., et al.: "Immunohistological distribution of antigenic determinants YH206 detected by a monoclonal antibody." Jpn. J. Med.25. 127-134 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yachi,A., et al.: "Innunological analysis of amyloid protein A with monoclonal antibodies." Protides of Biological Fluids. 34. 355-358 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kitagawa,A., Yachi,A., et al.: "Characterization of carcinoembryonic antigen-specific monoclonal antibodies and specific CEA assay in sera of patients." Jpn. J. Cancer Res. (Gann). 77. 922-930 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Sugiyama,T., Yachi,A., et al.: "Preparation of human monoclonal antibodies of IgG type to gastro-intestinal cancer-associated antigen." Jpn. J. Cancer Res. (gann). 77. 722-729 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yachi,A., Imai,K., et al.: "Human monoclonal antibodies directed to gastro-intestinal cancer associated antigens." Labeled Antibodies ( Kano,K. ed., Tokyo University Press, Tokyo ). 429-432 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Imai,K., Yachi,A., et al.: "Adenocarcinoma-associated YH206 antigen in the sera of cancer patients." Labeled Antibodies ( Kano,K. ed., Tokyo University Press, Tokyo ). 433-436 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Imai,K., Hinoda., et al.: "Application of monoclonal antibody KH2 against lactosyl ceramide and neolactotetraosyl ceramide to immunohistochemical study of digestive tract cancers." Gastroenterology. in press (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hinoda,Y., et al.: "A sandwich enzyme immunoassay of an adenocarcinoma-associated antigen YH206 in cancer sera." Jpn. J. Cancer Res. (Gann). in press (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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