| Project/Area Number |
60480214
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TAKINO Tatsuro Kyoto Prefectural University of Medicine, Professor, 医学部, 教授 (80079805)
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Toshiaki Kyoto Prefectural University of Medicine, Research assistant, 医学部, 助手 (00150574)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | Surface-coiled <^(31)P> NMR / Energy Metabolism / Rat Liver / Carbon Tetrachloride / Insulin / Glucagon / Taurine / Organ-reflectance Spectrophotometer |
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| Research Abstract |
The sequential changes in <^(31)P> -NMR spectra of rat experimental injured liver were determined by NMR spectroscopy (JEOL-SCM-200) equipped with surface-coil (NM-G27 TSPW). Simultaneous <^(31)P> - and <^1H> -NMR spectroscopic analyses on <CCl_4> -intoxicated rat liver in situ revealed that fatty infiltration in the liver preceded the depletion of hepatic ATP. In isolated perfused liver from <CCl_4> -treated rat, after an anoxic attack, the ATP level was restored incompletely. This suggests that mitochondrial oxidative phosphorylation system is damaged in <CCl_4> -intoxicated liver.
Insulin or glucagon decreased the ATP level in normal rat perfused liver, while simultaneous administration of these hormones prevented the decrement of hepatic ATP. In <CCl_4> -treated rat, not only glucagon but a combination of glucagon and insulin decreased the hepatic ATP level. The dose of glucagon may be a point in G-I therapy on hepatic failure. It was also revealed that taurine had a protective effect on anoxia induced ATP depletion of the liver.
On the other hand, the regional hepatic oxygen consumption and the reduction of mitochondrial cytochromes following anoxia were able to be assessed in both in vivo and isolated perfused liver by an organ-reflectance spectrophotometer (MCPD-100). The ICG clearance in rat liver in vivo were also measured. The change of reflectance spectra of the liver after ICG administration were constituted by three phases. The inflection points between the three phases are suggested to be useful for studying the hepatic ICG clearance in various liver diseases.
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