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Cloning of encephalitogenic T cells and analysis of immune mechanisms and treatment of experimental autoimmune encephalomyelitis.

Research Project

Project/Area Number 60480225
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionNational Center of Neurology and Psychiatry (N C N P) (1986)
国立武蔵療養所 (1985)

Principal Investigator

TABIRA Takeshi  National Institute of Neuroscience, National Center of Neurology and Psychiatry, その他, その他 (80112332)

Co-Investigator(Kenkyū-buntansha) KUNISHITA Tatsuhide  same, 神経センター神経研究所疾病研究第6部, 研究員 (40167383)
NAMIKAWA Tadashi  same, 神経センター神経研究所疾病研究第6部, 研究員 (30128061)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1986: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1985: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsExperimental allergic encephalomyelitis / Autoimmune / Myelin basic protein / Myelin proteolipid apoprotein / Demyelination / 多発性硬化症
Research Abstract

1. Finding of a novel encephalitogen.
It is well known that myelin basic protein (MBP) is an encephalitogen of experimental allergic encephalomyelitis (EAE). Myelin proteolipid apoprotein (PLP), a major protein component of central nerve myelin, was thought to be encephalitogenic in 1950s. However, it was denied because of contamination with MBP. This study has proven that PLP is a definite encephalitogen in guinea pigs, rats and mice. We have also shown that DM-20, a component of PLP, induces chronic relapsing EAE with widespread demyelination in BALB/c mice and T cell lines specific for PLP induce acute and relapsing EAE in SJL/J mice. These findings significantly contributed to the understanding of autoimmune encephalomyelitis especially of multiple sclerosis.
2. Analysis of immune mechanisms of EAE using T cell lines and clones.
MBP-spepcific encephalitogenic T cell lines and clones were established and used for analysis of cellular mechanism of EAE. We found that (1) a single T cell clone is enough to induce full-blown EAE in nude mice, (2) Ia antigens are expressed in the central nervous system lesions at acute and relapsed stage, and (3) an encephalitogenic T cell clone can be activated by allo-antigens and induces acute EAE.
3. Analysis of recovery mechanism and tolerance.
In order to develope new immunological treatment, we have studied recovery mechanism of acute EAE in Lewis rats. Self-limited ability of encephalitogenic T cells to continuous antigenic stimulation and environmental factors seem to be involved in the suppression. Suppression of EAE with antiserum to encephalitogenic T cell clone is now under study.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Yamamura T;Namikawa T;Endoh M;Kunishita T;Tabira T: Journal of Neuroimmunology. 12. 143-153 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yamamura T;Namikawa T;Endoh M;Kunishita T;Tabira T: Journal of Neurological Sciences. 76. 269-275 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Endoh M;Tabira T;Kunishita T;Sakai K;Yamamura T;Taketomi T: Journal of Immunology. 137. 3832-3835 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Namikawa T;Yamamura T;Sakai K;Kunishita T;Tabira T: International Archives of Allergy and Applied Immunology. 79. 370-375 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Sakai K;Tabira T;Endoh M;Steinman L: Laboratory Investigation. 54. 345-352 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Sakai K;Namikawa T;Kunishita T;Yamanouchi K;Tabira T: Journal of Immunology. 137. 1527-1531 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yamamura T, Namikawa T, Endoh M, Kunishita T, Tabira T: "Experimental allergic encephalomyelitis induced by proteolipid apoprotein in Lewis rats." Journal of Neuroimmunology. 12. 143-153 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yamamura T, Namikawa T, Endoh M, Kunishta T, Tabira T: "Passive transfer of experimental allergic encephalomyelitis induced by proteolipid apoprotein." Journal of the Neurological Sciences. 76. 269-275 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Endoh M, Tabira T, Kunishita T, Sakai K, Yamamura T, Taketomi T: "DM-20, a proteolipid apoprotein, is an encephalitogen of acute and relapsing autoimmune encephalomyelitis in mice." Journal of Immunology. 137. 3832-3835 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Namikawa T, Yamamura T, Sakai K, Kunishita T, Tabira T: "Activation of effector cells of experimental allergic encephalomyelitis in Lewis rats: comparison of T-cell lines with primary cultured lymph node cells." International Archives of Allergy and Applied Immunology. 79. 370-375 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Sakai K, Tabira T, Endoh M, Steinman L: "Ia expression in chronic relapsing experimental allergic encephalomyelitis induced by long-term cultured T cell lines in mice." Laboratory Investigation. 54. 345-352 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Sakai K, Namikawa T, Kunishita T, Yamanouchi K, Tabira T: "Studies of experimental allergic encephalomyelitis by using encephalitogenic T cell lines and clones in euthymic and athymic mice." Journal of Immunology. 137. 1527-1531 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Namikawa T, Kunishita T, Tabira T: "Modulation of experimental allergic encephalomyelitis (EAE): suppression of active reinduction of EAE in rats recovered from passively transferred disease." Journal of Neuroimmunology. 12. 235-245 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Satoh J, Sakai K, Endoh M, Koike F, Kunishita T, Namikawa T, Yamamura T, Tabira T: "Experimental allergic encephalomyelitis mediated by murine encephalitogenic T cell lines specific for myelin proteolipid apoprotein." Journal of Immunology. 138. 179-184 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Tabira T, Sakai K: "Demyelination induced by T cell lines and clones specific for myelin basic protein in mice." Laboratory Investigation. in press (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Namikawa T, Satoh J, Yamamura T, Sakai K, Kunishita T, Tabira T: "Recovery mechanisms from experimental allergic encephalomyelitis in rats: analysis by using encephalitogenic T cell lines." International Archives of Allergy and Applied Immunology. in press (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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