| Project/Area Number |
60480228
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | University of Tokyo |
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Principal Investigator |
YAZAKI YOSHIO University of Tokyo,Lacturer, 医学部, 講師 (20101090)
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| Co-Investigator(Kenkyū-buntansha) |
TSUCHIMOCHI HIDETSUGU University of Tokyo,Medical Staff, 医学部, 医員 (90197715)
ISOBE MITSUAKI University of Tokyo,Assistant, 医学部, 助手 (80176263)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1986: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1985: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | cardiac myosin / monoclonal antibody / myosin isozymes / Fetal type cardiac myosin / 梗塞画像診断 / 右室梗塞 |
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| Research Abstract |
We have developed anticardiac myosin antibodies, especially monoclonal antibodies for use in the diagnosis of heart disease. Our investigation are divided into two research projects. In the first we visualized the distribution of myosin isozymes in human atrial and ventricular myocardium by an immunofluorescence staining method using monoclonal antibodies specific for individual human cardiac myosin isozymes. We also revealed the redistribution of these cardiac myosin isozymes in an overloaded condition. The isozymic pattern of cardiac myosin was changed from atrial type to ventricular type in the overloaded atrium. This isozymic redistribution can be considered as an physiological adaptive mechanism to meet increased cardiac work during overload. In the second project we developed a new method for imaging myocardial infarction by single photon emission tomography using labeled monoclonal antibody specific for the cardiac myosin heavy chain . Specific localization of the labeled antibody was demonstrated in an infarcted area and no accumulation of radioactivity was shown in the bone matrix as observed in Tc-99m pyrophosphate images.
We also established a very sensitive microassay using antibodies which specifically bind the light chains liberated from the myocyte into serum following ischemic events, and developed a method for biochemically diagnosing myocardial infarction. Now that we can obtain accurate information regarding the size of the infarct which would be otherwise impossible by the conventional method, a wide range of clinical application is expected for this method.
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