| Project/Area Number |
60480243
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Yamanashi Medical College, Dept. of Pediatrics |
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Principal Investigator |
KATO Kiyohiko Yamanashi Medical College, Dept. of Ped., Professor, 小児科学教室, 教授 (80051069)
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| Co-Investigator(Kenkyū-buntansha) |
辻本 愛子 山梨医科大学, 小児科学教室, 医員
YOSHIKO Nakagomi Yamanashi Medicol College, Dept. of Ped.,, 小児科学教室, 助手 (20198055)
KOSUKE Higashida Yamanashi Medical College, Dept.of Ped.,, 小児科学教室, 助手 (60173142)
SHIN Amemiya Yamanashi Medical College, Dept. of Ped.,, 小児科学教室, 講師 (10118903)
AIKO Tsujimoto Yamanashi Medical College, Dept. of Ped.,
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1985: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | hepatic glycogen phosphorylase / adrenergic regulation / propranolol treatment in pregnancy / neonatal hypoglycemia / ホスホエノールピルベートカルボキシキナーゼ / ホスホエノールピルベートカルボキシナーゼ / グリコーゲンホスホリラーゼ / 細胞内遊離カルシウム / 単離肝細胞 |
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| Research Abstract |
1. The effect of development on adrenergic regulation of glycogenolysis were studied in rat liver. In isolated hepatocytes, the activation of glycogen phosphorylase by <alpha> -adrenergic stimulation decreased moderately with advancing age. However, activation by <beta> -adrenergic receptors more markedly declined and almost disappeared in isolate hepatocytes from 6- month-old rat.
The age-related changes in the pattern of stimulation of glycogen phos phorylase by catecholamines in isolated hepatocytes were accompanied by parallels changes in the numbers of <alpha>_1-and <beta> -adrenoceptors in membranes prepared from the isolated hepatocytes. A progressive decrease in the to tal number of <alpha>_1-receptors measured with (3H)-prazosin and <beta> -receptors measured with (126I) cyanopinadolol(CYP) was found with increasing age. The loss of <beta>-adrenergic receptors was much more dramatic. Our results suggest that age-related alterations of hepatic glycogen phosphorylase activetion by catecholamines may in part be explained by the changes in the ex-pression adrenergic receptors.
2. Postnatal increase of PEPCK(Phosphoenolpyruvate Carboxykinase) activity and the effect of maternal propranolol treatment on it were studied in rat liver. The activity of PEPCK rapidly increased after birth in rats, normal and surgical delivery. However, there was no significant difference in blood glucose level or PEPCK activity between control and propranolol treated group, which suggested that propranolol may not have significant effect on PEPCK induction in newborn rats and also, overall glucose meta bolism during neonatal period was not affected by <beta> -adrenergic system inhibition.
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